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10.1038/s41467-021-21627-0 http://scihub22266oqcxt.onion/10.1038/s41467-021-21627-0 33674580!7935920!33674580     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2021 ; 12 (1): 1467 Nephropedia Template TP {{tp|p=33674580|t=2021. Point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP |pdf=|usr=}}{{33674580}} gab.com Text Point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=33674580 #FOAvip Efforts to contain the spread of SARS-CoV-2 have spurred the need for reliable, rapid, and cost-effective diagnostic methods which can be applied to large numbers of people. However, current standard protocols for the detection of viral nucleic acids while sensitive, require a high level of automation and sophisticated laboratory equipment to achieve throughputs that allow whole communities to be tested on a regular basis. Here we present Cap-iLAMP (capture and improved loop-mediated isothermal amplification) which combines a hybridization capture-based RNA extraction of gargle lavage samples with an improved colorimetric RT-LAMP assay and smartphone-based color scoring. Cap-iLAMP is compatible with point-of-care testing and enables the detection of SARS-CoV-2 positive samples in less than one hour. In contrast to direct addition of the sample to improved LAMP (iLAMP), Cap-iLAMP prevents false positives and allows single positive samples to be detected in pools of 25 negative samples, reducing the reagent cost per test to ~1 Euro per individual. Twit Text FOAVip Point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=33674580 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33674580 #FOAvip English Wikipedia <ref>{{Cite pmid|33674580}}</ref> Point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP #MMPMID33674580 Bokelmann L; Nickel O; Maricic T; Paabo S; Meyer M; Borte S; Riesenberg S Nat Commun 2021[Mar]; 12 (1): 1467 PMID33674580show ga Efforts to contain the spread of SARS-CoV-2 have spurred the need for reliable, rapid, and cost-effective diagnostic methods which can be applied to large numbers of people. However, current standard protocols for the detection of viral nucleic acids while sensitive, require a high level of automation and sophisticated laboratory equipment to achieve throughputs that allow whole communities to be tested on a regular basis. Here we present Cap-iLAMP (capture and improved loop-mediated isothermal amplification) which combines a hybridization capture-based RNA extraction of gargle lavage samples with an improved colorimetric RT-LAMP assay and smartphone-based color scoring. Cap-iLAMP is compatible with point-of-care testing and enables the detection of SARS-CoV-2 positive samples in less than one hour. In contrast to direct addition of the sample to improved LAMP (iLAMP), Cap-iLAMP prevents false positives and allows single positive samples to be detected in pools of 25 negative samples, reducing the reagent cost per test to ~1 Euro per individual. |*Point-of-Care Testing[MESH] |COVID-19 Testing/*methods[MESH] |COVID-19/*diagnosis/*virology[MESH] |Colorimetry/*methods[MESH] |Coronavirus Nucleocapsid Proteins/genetics[MESH] |Humans[MESH] |Molecular Diagnostic Techniques/*methods[MESH] |Nucleic Acid Amplification Techniques/*methods[MESH] |Nucleic Acid Hybridization/*methods[MESH] |Phosphoproteins/genetics[MESH] |RNA, Viral/genetics[MESH] |SARS-CoV-2/genetics/*isolation & purification[MESH]Point-of-care bulk testing for SARS-CoV-2 by combining hybridization c(epmc).pdf DeepDyve Pubget Overpricing