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10.3390/bioengineering8020023 http://scihub22266oqcxt.onion/10.3390/bioengineering8020023 33673107!7918862!33673107     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Bioengineering+(Basel) 2021 ; 8 (2): ä Nephropedia Template TP {{tp|p=33673107|t=2021. CRISPR/Cas9-Based Lateral Flow and Fluorescence Diagnostics |pdf=|usr=}}{{33673107}} gab.com Text CRISPR/Cas9-Based Lateral Flow and Fluorescence Diagnostics JO: Bioengineering (Basel) http://www.kidney.de/pget.php?&tw=1&pmid=33673107 #FOAvip Clustered regularly interspaced short palindromic repeat (CRISPR/Cas) proteins can be designed to bind specified DNA and RNA sequences and hold great promise for the accurate detection of nucleic acids for diagnostics. We integrated commercially available reagents into a CRISPR/Cas9-based lateral flow assay that can detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) sequences with single-base specificity. This approach requires minimal equipment and represents a simplified platform for field-based deployment. We also developed a rapid, multiplex fluorescence CRISPR/Cas9 nuclease cleavage assay capable of detecting and differentiating SARS-CoV-2, influenza A and B, and respiratory syncytial virus in a single reaction. Our findings provide proof-of-principle for CRISPR/Cas9 point-of-care diagnosis as well as a scalable fluorescent platform for identifying respiratory viral pathogens with overlapping symptomology. Twit Text FOAVip CRISPR/Cas9-Based Lateral Flow and Fluorescence Diagnostics ????Bioengineering (Basel) http://www.kidney.de/pget.php?&tw=1&pmid=33673107 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33673107 #FOAvip English Wikipedia <ref>{{Cite pmid|33673107}}</ref> CRISPR/Cas9-Based Lateral Flow and Fluorescence Diagnostics #MMPMID33673107 Osborn MJ; Bhardwaj A; Bingea SP; Knipping F; Feser CJ; Lees CJ; Collins DP; Steer CJ; Blazar BR; Tolar J Bioengineering (Basel) 2021[Feb]; 8 (2): ä PMID33673107show ga Clustered regularly interspaced short palindromic repeat (CRISPR/Cas) proteins can be designed to bind specified DNA and RNA sequences and hold great promise for the accurate detection of nucleic acids for diagnostics. We integrated commercially available reagents into a CRISPR/Cas9-based lateral flow assay that can detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) sequences with single-base specificity. This approach requires minimal equipment and represents a simplified platform for field-based deployment. We also developed a rapid, multiplex fluorescence CRISPR/Cas9 nuclease cleavage assay capable of detecting and differentiating SARS-CoV-2, influenza A and B, and respiratory syncytial virus in a single reaction. Our findings provide proof-of-principle for CRISPR/Cas9 point-of-care diagnosis as well as a scalable fluorescent platform for identifying respiratory viral pathogens with overlapping symptomology. äCRISPR/Cas9-Based Lateral Flow and Fluorescence Diagnostics (epmc).pdf DeepDyve Pubget Overpricing