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10.3390/pathogens10020195 http://scihub22266oqcxt.onion/10.3390/pathogens10020195 33673032!7917696!33673032     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Pathogens 2021 ; 10 (2): ä Nephropedia Template TP {{tp|p=33673032|t=2021. Evaluation of Two Methods to Concentrate SARS-CoV-2 from Untreated Wastewater |pdf=|usr=}}{{33673032}} gab.com Text Evaluation of Two Methods to Concentrate SARS-CoV-2 from Untreated Wastewater JO: Pathogens http://www.kidney.de/pget.php?&tw=1&pmid=33673032 #FOAvip Use of wastewater-based epidemiology as a tool to record and manage the course of SARS-CoV-2 infections in human populations requires information about the efficiency of methods to concentrate the virus from wastewater. In the present study, we spiked untreated wastewater with quantified SARS-CoV-2 positive clinical material and enriched the virus by polyethylene glycol precipitation and ultrafiltration with Vivaspin 10 kDa MWCO columns. SARS-CoV-2 was detected and quantified by reverse transcription quantitative PCR (E- and S-gene) and droplet digital PCR. The concentration of virus with precipitation resulted in mean recoveries between 59.4% and 63.7% whereas rates from 33.0% to 42.6% after ultrafiltration of samples were demonstrated. The results suggest that the use of both methods allows an effective and practicable enrichment of SARS-CoV-2 from raw wastewater. Twit Text FOAVip Evaluation of Two Methods to Concentrate SARS-CoV-2 from Untreated Wastewater ????Pathogens http://www.kidney.de/pget.php?&tw=1&pmid=33673032 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33673032 #FOAvip English Wikipedia <ref>{{Cite pmid|33673032}}</ref> Evaluation of Two Methods to Concentrate SARS-CoV-2 from Untreated Wastewater #MMPMID33673032 Dumke R; de la Cruz Barron M; Oertel R; Helm B; Kallies R; Berendonk TU; Dalpke A Pathogens 2021[Feb]; 10 (2): ä PMID33673032show ga Use of wastewater-based epidemiology as a tool to record and manage the course of SARS-CoV-2 infections in human populations requires information about the efficiency of methods to concentrate the virus from wastewater. In the present study, we spiked untreated wastewater with quantified SARS-CoV-2 positive clinical material and enriched the virus by polyethylene glycol precipitation and ultrafiltration with Vivaspin 10 kDa MWCO columns. SARS-CoV-2 was detected and quantified by reverse transcription quantitative PCR (E- and S-gene) and droplet digital PCR. The concentration of virus with precipitation resulted in mean recoveries between 59.4% and 63.7% whereas rates from 33.0% to 42.6% after ultrafiltration of samples were demonstrated. The results suggest that the use of both methods allows an effective and practicable enrichment of SARS-CoV-2 from raw wastewater. äEvaluation of Two Methods to Concentrate SARS-CoV-2 from Untreated Was(epmc).pdf DeepDyve Pubget Overpricing