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10.1016/j.pep.2021.105861 http://scihub22266oqcxt.onion/10.1016/j.pep.2021.105861 33667651!7923943!33667651     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=33667651&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215       Protein+Expr+Purif 2021 ; 183 (?): 105861 Nephropedia Template TP {{tp|p=33667651|t=2021. Expression of SARS-CoV-2 surface glycoprotein fragment 319-640 in E coli, and its refolding and purification |pdf=|usr=}}{{33667651}} gab.com Text Expression of SARS-CoV-2 surface glycoprotein fragment 319-640 in E coli, and its refolding and purification JO: Protein Expr Purif http://www.kidney.de/pget.php?&tw=1&pmid=33667651 #FOAvip Sensitive and specific serology tests are essential for epidemiological and public health studies of COVID-19 and for vaccine efficacy testing. The presence of antibodies to SARS-CoV-2 surface glycoprotein (Spike) and, specifically, its receptor-binding domain (RBD) correlates with inhibition of SARS-CoV-2 binding to the cellular receptor and viral entry into the cells. Serology tests that detect antibodies targeting RBD have high potential to predict COVID-19 immunity and to accurately determine the extent of the vaccine-induced immune response. Cost-effective methods of expression and purification of Spike and its fragments that preserve antigenic properties are essential for development of such tests. Here we describe a method of production of His6-tagged S319-640 fragment containing RBD in E. coli. It includes expression of the fragment, solubilization of inclusion bodies, and on-the-column refolding. The antigenic properties of the resulting product are similar, but not identical to the RBD-containing fragment expressed in human cells. Twit Text FOAVip Expression of SARS-CoV-2 surface glycoprotein fragment 319-640 in E coli, and its refolding and purification ????Protein Expr Purif http://www.kidney.de/pget.php?&tw=1&pmid=33667651 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33667651 #FOAvip English Wikipedia <ref>{{Cite pmid|33667651}}</ref> Expression of SARS-CoV-2 surface glycoprotein fragment 319-640 in E coli, and its refolding and purification #MMPMID33667651 Fitzgerald GA; Komarov A; Kaznadzey A; Mazo I; Kireeva ML Protein Expr Purif 2021[Jul]; 183 (?): 105861 PMID33667651show ga Sensitive and specific serology tests are essential for epidemiological and public health studies of COVID-19 and for vaccine efficacy testing. The presence of antibodies to SARS-CoV-2 surface glycoprotein (Spike) and, specifically, its receptor-binding domain (RBD) correlates with inhibition of SARS-CoV-2 binding to the cellular receptor and viral entry into the cells. Serology tests that detect antibodies targeting RBD have high potential to predict COVID-19 immunity and to accurately determine the extent of the vaccine-induced immune response. Cost-effective methods of expression and purification of Spike and its fragments that preserve antigenic properties are essential for development of such tests. Here we describe a method of production of His6-tagged S319-640 fragment containing RBD in E. coli. It includes expression of the fragment, solubilization of inclusion bodies, and on-the-column refolding. The antigenic properties of the resulting product are similar, but not identical to the RBD-containing fragment expressed in human cells. |Binding Sites[MESH] |COVID-19/*virology[MESH] |Cloning, Molecular[MESH] |Escherichia coli/chemistry/genetics[MESH] |Gene Expression[MESH] |Humans[MESH] |Peptide Fragments/chemistry/genetics/isolation & purification[MESH] |Protein Domains[MESH] |Protein Refolding[MESH] |SARS-CoV-2/*chemistry/genetics[MESH] |Solubility[MESH]Expression of SARS-CoV-2 surface glycoprotein fragment 319-640 in E c(epmc).pdf DeepDyve Pubget Overpricing