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10.1111/boc.202000158 http://scihub22266oqcxt.onion/10.1111/boc.202000158 33666950!8014678!33666950     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biol+Cell 2021 ; 113 (7): 311-328 Nephropedia Template TP {{tp|p=33666950|t=2021. A comprehensive library of fluorescent constructs of SARS-CoV-2 proteins and their initial characterisation in different cell types |pdf=|usr=}}{{33666950}} gab.com Text A comprehensive library of fluorescent constructs of SARS-CoV-2 proteins and their initial characterisation in different cell types JO: Biol Cell http://www.kidney.de/pget.php?&tw=1&pmid=33666950 #FOAvip BACKGROUND INFORMATION: Comprehensive libraries of plasmids for SARS-CoV-2 proteins with various tags (e.g., Strep, HA, Turbo) are now available. They enable the identification of numerous potential protein-protein interactions between the SARS-CoV-2 virus and host proteins. RESULTS: We present here a large library of SARS CoV-2 protein constructs fused with green and red fluorescent proteins and their initial characterisation in various human cell lines including lung epithelial cell models (A549, BEAS-2B), as well as in budding yeast. The localisation of a few SARS-CoV-2 proteins matches their proposed interactions with host proteins. These include the localisation of Nsp13 to the centrosome, Orf3a to late endosomes and Orf9b to mitochondria. CONCLUSIONS AND SIGNIFICANCE: This library should facilitate further cellular investigations, notably by imaging techniques. Twit Text FOAVip A comprehensive library of fluorescent constructs of SARS-CoV-2 proteins and their initial characterisation in different cell types ????Biol Cell http://www.kidney.de/pget.php?&tw=1&pmid=33666950 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33666950 #FOAvip English Wikipedia <ref>{{Cite pmid|33666950}}</ref> A comprehensive library of fluorescent constructs of SARS-CoV-2 proteins and their initial characterisation in different cell types #MMPMID33666950 Miserey-Lenkei S; Trajkovic K; D'Ambrosio JM; Patel AJ; Copic A; Mathur P; Schauer K; Goud B; Albanese V; Gautier R; Subra M; Kovacs D; Barelli H; Antonny B Biol Cell 2021[Jul]; 113 (7): 311-328 PMID33666950show ga BACKGROUND INFORMATION: Comprehensive libraries of plasmids for SARS-CoV-2 proteins with various tags (e.g., Strep, HA, Turbo) are now available. They enable the identification of numerous potential protein-protein interactions between the SARS-CoV-2 virus and host proteins. RESULTS: We present here a large library of SARS CoV-2 protein constructs fused with green and red fluorescent proteins and their initial characterisation in various human cell lines including lung epithelial cell models (A549, BEAS-2B), as well as in budding yeast. The localisation of a few SARS-CoV-2 proteins matches their proposed interactions with host proteins. These include the localisation of Nsp13 to the centrosome, Orf3a to late endosomes and Orf9b to mitochondria. CONCLUSIONS AND SIGNIFICANCE: This library should facilitate further cellular investigations, notably by imaging techniques. |*Peptide Library[MESH] |A549 Cells[MESH] |COVID-19/*virology[MESH] |Cell Line[MESH] |Green Fluorescent Proteins/genetics/metabolism[MESH] |Host Microbial Interactions/physiology[MESH] |Humans[MESH] |Luminescent Proteins/genetics/metabolism[MESH] |Microscopy, Fluorescence[MESH] |Protein Interaction Domains and Motifs[MESH] |Recombinant Fusion Proteins/genetics/metabolism[MESH] |Red Fluorescent Protein[MESH] |SARS-CoV-2/genetics/*metabolism[MESH] |Saccharomyces cerevisiae/genetics/metabolism[MESH] |Time-Lapse Imaging[MESH]A comprehensive library of fluorescent constructs of SARS-CoV-2 protei(epmc).pdf DeepDyve Pubget Overpricing