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suck abstract from ncbi


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pmid33666905      Med+J+(Ft+Sam+Houst+Tex) 2021 ; ä (PB 8-21-01/02/03): 8-11
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  • Pooling Nasopharyngeal Swab Specimens to Increase Testing Capacity for SARS-CoV-2 #MMPMID33666905
  • Anderson C; Castillo F; Koenig M; Managbanag JR
  • Med J (Ft Sam Houst Tex) 2021[Jan]; ä (PB 8-21-01/02/03): 8-11 PMID33666905show ga
  • The recent emergence of SARS-CoV-2 has led to a global pandemic of unprecedented proportions. Current diagnosis of COVID-19 relies on the detection of SARS-CoV-2 RNA by reverse transcription polymerase chain reaction (RT-PCR) in upper and lower respiratory specimens. While sensitive and specific, these RT-PCR assays require considerable supplies and reagents, which are often limited during global pandemics and surge testing. Here, we show that a nasopharyngeal swab pooling strategy can detect a single positive sample in pools of up to 10 samples without sacrificing RT-PCR sensitivity and specificity. We also report that this pooling strategy can be applied to rapid, moderate complexity assays, such as the BioFire COVID-19 test. Implementing a pooling strategy can significantly increase laboratory testing capacity while simultaneously reducing turnaround times for rapid identification and isolation of positive COVID-19 cases in high risk populations.
  • |*COVID-19 Nucleic Acid Testing[MESH]
  • |*Reverse Transcriptase Polymerase Chain Reaction[MESH]
  • |*Specimen Handling[MESH]
  • |COVID-19/*diagnosis[MESH]
  • |Humans[MESH]
  • |Nasopharynx/virology[MESH]
  • |RNA, Viral/*isolation & purification[MESH]
  • |SARS-CoV-2/*isolation & purification[MESH]


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