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10.1111/tmi.13569 http://scihub22266oqcxt.onion/10.1111/tmi.13569 33666297!8014856!33666297     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Trop+Med+Int+Health 2021 ; 26 (6): 621-631 Nephropedia Template TP {{tp|p=33666297|t=2021. Limited specificity of commercially available SARS-CoV-2 IgG ELISAs in serum samples of African origin |pdf=|usr=}}{{33666297}} gab.com Text Limited specificity of commercially available SARS-CoV-2 IgG ELISAs in serum samples of African origin JO: Trop Med Int Health http://www.kidney.de/pget.php?&tw=1&pmid=33666297 #FOAvip OBJECTIVES: Specific serological tests are mandatory for reliable SARS-CoV-2 diagnostics and seroprevalence studies. Here, we assess the specificities of four commercially available SARS-CoV-2 IgG ELISAs in serum/plasma panels originating from Africa, South America, and Europe. METHODS: 882 serum/plasma samples collected from symptom-free donors before the COVID-19 pandemic in three African countries (Ghana, Madagascar, Nigeria), Colombia, and Germany were analysed with three nucleocapsid-based ELISAs (Euroimmun Anti-SARS-CoV-2-NCP IgG, EDI Novel Coronavirus COVID-19 IgG, Mikrogen recomWell SARS-CoV-2 IgG), one spike/S1-based ELISA (Euroimmun Anti-SARS-CoV-2 IgG), and in-house common cold CoV ELISAs. RESULTS: High specificity was confirmed for all SARS-CoV-2 IgG ELISAs for Madagascan (93.4-99.4%), Colombian (97.8-100.0%), and German (95.9-100.0%) samples. In contrast, specificity was much lower for the Ghanaian and Nigerian serum panels (Ghana: NCP-based assays 77.7-89.7%, spike/S1-based assay 94.3%; Nigeria: NCP-based assays 39.3-82.7%, spike/S1-based assay 90.7%). 15 of 600 African sera were concordantly classified as positive in both the NCP-based and the spike/S1-based Euroimmun ELISA, but did not inhibit spike/ACE2 binding in a surrogate virus neutralisation test. IgG antibodies elicited by previous infections with common cold CoVs were found in all sample panels, including those from Madagascar, Colombia, and Germany and thus do not inevitably hamper assay specificity. Nevertheless, high levels of IgG antibodies interacting with OC43 NCP were found in all 15 SARS-CoV-2 NCP/spike/S1 ELISA positive sera. CONCLUSIONS: Depending on the chosen antigen and assay protocol, SARS-CoV-2 IgG ELISA specificity may be significantly reduced in certain populations probably due to interference of immune responses to endemic pathogens like other viruses or parasites. Twit Text FOAVip Limited specificity of commercially available SARS-CoV-2 IgG ELISAs in serum samples of African origin ????Trop Med Int Health http://www.kidney.de/pget.php?&tw=1&pmid=33666297 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33666297 #FOAvip English Wikipedia <ref>{{Cite pmid|33666297}}</ref> Limited specificity of commercially available SARS-CoV-2 IgG ELISAs in serum samples of African origin #MMPMID33666297 Emmerich P; Murawski C; Ehmen C; von Possel R; Pekarek N; Oestereich L; Duraffour S; Pahlmann M; Struck N; Eibach D; Krumkamp R; Amuasi J; Maiga-Ascofare O; Rakotozandrindrainy R; Asogun D; Ighodalo Y; Kann S; May J; Tannich E; Deschermeier C Trop Med Int Health 2021[Jun]; 26 (6): 621-631 PMID33666297show ga OBJECTIVES: Specific serological tests are mandatory for reliable SARS-CoV-2 diagnostics and seroprevalence studies. Here, we assess the specificities of four commercially available SARS-CoV-2 IgG ELISAs in serum/plasma panels originating from Africa, South America, and Europe. METHODS: 882 serum/plasma samples collected from symptom-free donors before the COVID-19 pandemic in three African countries (Ghana, Madagascar, Nigeria), Colombia, and Germany were analysed with three nucleocapsid-based ELISAs (Euroimmun Anti-SARS-CoV-2-NCP IgG, EDI Novel Coronavirus COVID-19 IgG, Mikrogen recomWell SARS-CoV-2 IgG), one spike/S1-based ELISA (Euroimmun Anti-SARS-CoV-2 IgG), and in-house common cold CoV ELISAs. RESULTS: High specificity was confirmed for all SARS-CoV-2 IgG ELISAs for Madagascan (93.4-99.4%), Colombian (97.8-100.0%), and German (95.9-100.0%) samples. In contrast, specificity was much lower for the Ghanaian and Nigerian serum panels (Ghana: NCP-based assays 77.7-89.7%, spike/S1-based assay 94.3%; Nigeria: NCP-based assays 39.3-82.7%, spike/S1-based assay 90.7%). 15 of 600 African sera were concordantly classified as positive in both the NCP-based and the spike/S1-based Euroimmun ELISA, but did not inhibit spike/ACE2 binding in a surrogate virus neutralisation test. IgG antibodies elicited by previous infections with common cold CoVs were found in all sample panels, including those from Madagascar, Colombia, and Germany and thus do not inevitably hamper assay specificity. Nevertheless, high levels of IgG antibodies interacting with OC43 NCP were found in all 15 SARS-CoV-2 NCP/spike/S1 ELISA positive sera. CONCLUSIONS: Depending on the chosen antigen and assay protocol, SARS-CoV-2 IgG ELISA specificity may be significantly reduced in certain populations probably due to interference of immune responses to endemic pathogens like other viruses or parasites. |Adolescent[MESH] |Adult[MESH] |Antibodies, Viral/*blood[MESH] |COVID-19/*diagnosis/virology[MESH] |Child[MESH] |Child, Preschool[MESH] |Colombia[MESH] |Coronavirus Nucleocapsid Proteins/immunology[MESH] |Enzyme-Linked Immunosorbent Assay/*methods[MESH] |Female[MESH] |Germany[MESH] |Ghana[MESH] |Humans[MESH] |Immunoglobulin G/*blood[MESH] |Madagascar[MESH] |Male[MESH] |Middle Aged[MESH] |Nigeria[MESH] |SARS-CoV-2/immunology/isolation & purification[MESH] |Sensitivity and Specificity[MESH] |Spike Glycoprotein, Coronavirus/immunology[MESH]Limited specificity of commercially available SARS-CoV-2 IgG ELISAs in(epmc).pdf DeepDyve Pubget Overpricing