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10.4155/bio-2021-0002 http://scihub22266oqcxt.onion/10.4155/bio-2021-0002 33661025!ä!33661025 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Bioanalysis 2021 ; 13 (5): 387-394 Nephropedia Template TP {{tp|p=33661025|t=2021. Real-time PCR assay as a simple and efficient tool for viral stability study |pdf=|usr=}}{{33661025}} gab.com Text Real-time PCR assay as a simple and efficient tool for viral stability study JO: Bioanalysis http://www.kidney.de/pget.php?&tw=1&pmid=33661025 #FOAvip Aim: For oncolytic virus trials, regulatory agencies often require pharmaceutical industry to evaluate risks of released viruses from patients to environment. This study was to establish a real-time PCR method to assess viral shedding and viral stability in human urine. Results/methodology: Herein, we describe an incubation of viral drug product in human urine and use of real-time PCR as a simple, efficient and high throughput assay to assess the level and stability of a nonenveloped and single stranded RNA virus. The viral stability issue is critical to the collection, transport, storage and testing of clinical samples. Discussion/conclusion: In summary, this simple method provides useful viral stability information at various temperatures and detergents. A similar approach may apply to other RNA viruses (including SARS-CoV-2). Twit Text FOAVip Real-time PCR assay as a simple and efficient tool for viral stability study ????Bioanalysis http://www.kidney.de/pget.php?&tw=1&pmid=33661025 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33661025 #FOAvip English Wikipedia <ref>{{Cite pmid|33661025}}</ref> Real-time PCR assay as a simple and efficient tool for viral stability study #MMPMID33661025 Hu B; Dopp JL; Salituro GM; Rubinstein LJ Bioanalysis 2021[Mar]; 13 (5): 387-394 PMID33661025show ga Aim: For oncolytic virus trials, regulatory agencies often require pharmaceutical industry to evaluate risks of released viruses from patients to environment. This study was to establish a real-time PCR method to assess viral shedding and viral stability in human urine. Results/methodology: Herein, we describe an incubation of viral drug product in human urine and use of real-time PCR as a simple, efficient and high throughput assay to assess the level and stability of a nonenveloped and single stranded RNA virus. The viral stability issue is critical to the collection, transport, storage and testing of clinical samples. Discussion/conclusion: In summary, this simple method provides useful viral stability information at various temperatures and detergents. A similar approach may apply to other RNA viruses (including SARS-CoV-2). |*Real-Time Polymerase Chain Reaction[MESH] |COVID-19/diagnosis/virology[MESH] |Detergents/chemistry[MESH] |Humans[MESH] |RNA Stability[MESH] |RNA, Viral/blood/*metabolism/urine[MESH] |SARS-CoV-2/genetics/isolation & purification[MESH] |Temperature[MESH]Real-time PCR assay as a simple and efficient tool for viral stability(epmc).pdf DeepDyve Pubget Overpricing