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10.21769/BioProtoc.3789

http://scihub22266oqcxt.onion/10.21769/BioProtoc.3789
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33659444!7842347!33659444
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suck abstract from ncbi


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pmid33659444      Bio+Protoc 2020 ; 10 (20): e3789
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  • A Protocol for Simple, Rapid, and Direct Detection of SARS-CoV-2 from clinical samples, using Reverse Transcribed Loop-Mediated Isothermal Amplification (RT-LAMP) #MMPMID33659444
  • Naddaf R; Ben-Assa N; Gefen T; Capucha T; Hajjo H; Mandelbaum N; Elbaum L; Kaplan S; Rotem A; Chowers M; Szwarcwort-Cohen M; Paul M; Geva-Zatorsky N
  • Bio Protoc 2020[Oct]; 10 (20): e3789 PMID33659444show ga
  • SARS-CoV-2 has quickly spread all around the globe causing illness and wide damages. Most countries were unprepared for such a rapid spread and crisis. This led to various strategies for effective control of the new pandemic. A key aspect in all countries was to effectively test the population for the virus. Most countries chose a lockdown strategy in which many workplaces and activities are completely closed, leading to substantial economy costs. Here, we present a protocol we recently developed that allows rapid and simple detection of SARS-CoV-2 for the large population, eliminating costs and involvement of professional teams and laboratories. This protocol is based on Reverse Transcribed Loop-Mediated Isothermal Amplification (RT-LAMP). We tested this protocol directly on patient samples, both nasal and throat clinical swabs as well as saliva. Notably, this protocol is simple, cheap and can be easily applied to other pathogens as well.
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