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10.1126/sciadv.abf1004 http://scihub22266oqcxt.onion/10.1126/sciadv.abf1004 33658206!7929516!33658206     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Adv 2021 ; 7 (10): ä Nephropedia Template TP {{tp|p=33658206|t=2021. Hallmarks of Alpha- and Betacoronavirus non-structural protein 7+8 complexes |pdf=|usr=}}{{33658206}} gab.com Text Hallmarks of Alpha- and Betacoronavirus non-structural protein 7+8 complexes JO: Sci Adv http://www.kidney.de/pget.php?&tw=1&pmid=33658206 #FOAvip Coronaviruses infect many different species including humans. The last two decades have seen three zoonotic coronaviruses, with SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) causing a pandemic in 2020. Coronaviral non-structural proteins (nsps) form the replication-transcription complex (RTC). Nsp7 and nsp8 interact with and regulate the RNA-dependent RNA-polymerase and other enzymes in the RTC. However, the structural plasticity of nsp7+8 complexes has been under debate. Here, we present the framework of nsp7+8 complex stoichiometry and topology based on native mass spectrometry and complementary biophysical techniques of nsp7+8 complexes from seven coronaviruses in the genera Alpha- and Betacoronavirus including SARS-CoV-2. Their complexes cluster into three groups, which systematically form either heterotrimers or heterotetramers or both, exhibiting distinct topologies. Moreover, even at high protein concentrations, SARS-CoV-2 nsp7+8 consists primarily of heterotetramers. From these results, the different assembly paths can be pinpointed to specific residues and an assembly model proposed. Twit Text FOAVip Hallmarks of Alpha- and Betacoronavirus non-structural protein 7+8 complexes ????Sci Adv http://www.kidney.de/pget.php?&tw=1&pmid=33658206 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33658206 #FOAvip English Wikipedia <ref>{{Cite pmid|33658206}}</ref> Hallmarks of Alpha- and Betacoronavirus non-structural protein 7+8 complexes #MMPMID33658206 Krichel B; Bylapudi G; Schmidt C; Blanchet C; Schubert R; Brings L; Koehler M; Zenobi R; Svergun D; Lorenzen K; Madhugiri R; Ziebuhr J; Uetrecht C Sci Adv 2021[Mar]; 7 (10): ä PMID33658206show ga Coronaviruses infect many different species including humans. The last two decades have seen three zoonotic coronaviruses, with SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) causing a pandemic in 2020. Coronaviral non-structural proteins (nsps) form the replication-transcription complex (RTC). Nsp7 and nsp8 interact with and regulate the RNA-dependent RNA-polymerase and other enzymes in the RTC. However, the structural plasticity of nsp7+8 complexes has been under debate. Here, we present the framework of nsp7+8 complex stoichiometry and topology based on native mass spectrometry and complementary biophysical techniques of nsp7+8 complexes from seven coronaviruses in the genera Alpha- and Betacoronavirus including SARS-CoV-2. Their complexes cluster into three groups, which systematically form either heterotrimers or heterotetramers or both, exhibiting distinct topologies. Moreover, even at high protein concentrations, SARS-CoV-2 nsp7+8 consists primarily of heterotetramers. From these results, the different assembly paths can be pinpointed to specific residues and an assembly model proposed. |Alphacoronavirus/*metabolism[MESH] |Amino Acid Sequence[MESH] |Betacoronavirus/*metabolism[MESH] |Conserved Sequence[MESH] |Cross-Linking Reagents/chemistry[MESH] |Models, Molecular[MESH] |Protein Multimerization[MESH] |Protein Subunits/metabolism[MESH] |Scattering, Radiation[MESH] |Scattering, Small Angle[MESH] |Species Specificity[MESH] |Viral Nonstructural Proteins/chemistry/*metabolism[MESH]Hallmarks of Alpha- and Betacoronavirus non-structural protein 7+8 com(epmc).pdf DeepDyve Pubget Overpricing