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10.1038/s41598-021-83950-2 http://scihub22266oqcxt.onion/10.1038/s41598-021-83950-2 33649400!7921094!33649400     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2021 ; 11 (1): 4904 Nephropedia Template TP {{tp|p=33649400|t=2021. Impact of SARS-CoV-2 infection on the recovery of peripheral blood mononuclear cells by density gradient |pdf=|usr=}}{{33649400}} gab.com Text Impact of SARS-CoV-2 infection on the recovery of peripheral blood mononuclear cells by density gradient JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=33649400 #FOAvip SARS-CoV-2 virus infection is responsible for coronavirus disease (COVID-19), which is characterised by a hyperinflammatory response that plays a major role in determining the respiratory and immune-mediated complications of this condition. While isolating peripheral blood mononuclear cells (PBMCs) from whole blood of COVID-19 patients by density gradient centrifugation, we noticed some changes in the floating properties and in the sedimentation of the cells on density medium. Investigating this further, we found that in early phase COVID-19 patients, characterised by reduced circulating lymphocytes and monocytes, the PBMC fraction contained surprisingly high levels of neutrophils. Furthermore, the neutrophil population exhibited alterations in the cell size and in the internal complexity, consistent with the presence of low density neutrophils (LDNs) and immature forms, which may explain the shift seen in the floating abilities and that may be predictive of the severity of the disease. The percentage of this subset of neutrophils found in the PBMC band was rather spread (35.4 +/- 27.2%, with a median 28.8% and IQR 11.6-56.1, Welch's t-test early phase COVID-19 versus blood donor healthy controls P < 0.0001). Results confirm the presence of an increased number of LDNs in patients with early stage COVID-19, which correlates with disease severity and may be recovered by centrifugation on a density gradient together with PBMCs. Twit Text FOAVip Impact of SARS-CoV-2 infection on the recovery of peripheral blood mononuclear cells by density gradient ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=33649400 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33649400 #FOAvip English Wikipedia <ref>{{Cite pmid|33649400}}</ref> Impact of SARS-CoV-2 infection on the recovery of peripheral blood mononuclear cells by density gradient #MMPMID33649400 Manunta MDI; Lamorte G; Ferrari F; Trombetta E; Tirone M; Bianco C; Cattaneo A; Santoro L; Baselli G; Brasca M; Ostadreza M; Erba E; Gori A; Bandera A; Porretti L; Valenti LVC; Prati D Sci Rep 2021[Mar]; 11 (1): 4904 PMID33649400show ga SARS-CoV-2 virus infection is responsible for coronavirus disease (COVID-19), which is characterised by a hyperinflammatory response that plays a major role in determining the respiratory and immune-mediated complications of this condition. While isolating peripheral blood mononuclear cells (PBMCs) from whole blood of COVID-19 patients by density gradient centrifugation, we noticed some changes in the floating properties and in the sedimentation of the cells on density medium. Investigating this further, we found that in early phase COVID-19 patients, characterised by reduced circulating lymphocytes and monocytes, the PBMC fraction contained surprisingly high levels of neutrophils. Furthermore, the neutrophil population exhibited alterations in the cell size and in the internal complexity, consistent with the presence of low density neutrophils (LDNs) and immature forms, which may explain the shift seen in the floating abilities and that may be predictive of the severity of the disease. The percentage of this subset of neutrophils found in the PBMC band was rather spread (35.4 +/- 27.2%, with a median 28.8% and IQR 11.6-56.1, Welch's t-test early phase COVID-19 versus blood donor healthy controls P < 0.0001). Results confirm the presence of an increased number of LDNs in patients with early stage COVID-19, which correlates with disease severity and may be recovered by centrifugation on a density gradient together with PBMCs. |*Cell Separation[MESH] |Adult[MESH] |COVID-19/*blood/pathology[MESH] |Centrifugation, Density Gradient[MESH] |Female[MESH] |Humans[MESH] |Leukocytes, Mononuclear/*metabolism/pathology[MESH] |Male[MESH] |Middle Aged[MESH]Impact of SARS-CoV-2 infection on the recovery of peripheral blood mon(epmc).pdf DeepDyve Pubget Overpricing