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10.1016/j.bios.2021.113099 http://scihub22266oqcxt.onion/10.1016/j.bios.2021.113099 33640656!7892303!33640656     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biosens+Bioelectron 2021 ; 179 (ä): 113099 Nephropedia Template TP {{tp|p=33640656|t=2021. Emulsion-based isothermal nucleic acid amplification for rapid SARS-CoV-2 detection via angle-dependent light scatter analysis |pdf=|usr=}}{{33640656}} gab.com Text Emulsion-based isothermal nucleic acid amplification for rapid SARS-CoV-2 detection via angle-dependent light scatter analysis JO: Biosens Bioelectron http://www.kidney.de/pget.php?&tw=1&pmid=33640656 #FOAvip The SARS-CoV-2 pandemic, an ongoing global health crisis, has revealed the need for new technologies that integrate the sensitivity and specificity of RT-PCR tests with a faster time-to-detection. Here, an emulsion loop-mediated isothermal amplification (eLAMP) platform was developed to allow for the compartmentalization of LAMP reactions, leading to faster changes in emulsion characteristics, and thus lowering time-to-detection. Within these droplets, ongoing LAMP reactions lead to adsorption of amplicons to the water-oil interface, causing a decrease in interfacial tension, resulting in smaller emulsion diameters. Changes in emulsion diameter allow for the monitoring of the reaction by use of angle-dependent light scatter (based off Mie scatter theory). Mie scatter simulations confirmed that light scatter intensity is diameter-dependent and smaller colloids have lower intensity values compared to larger colloids. Via spectrophotometers and fiber optic cables placed at 30 degrees and 60 degrees , light scatter intensity was monitored. Scatter intensities collected at 5 min, 30 degrees could statistically differentiate 10, 10(3), and 10(5) copies/muL initial concentrations compared to NTC. Similarly, 5 min scatter intensities collected at 60 degrees could statistically differentiate 10(5) copies/muL initial concentrations in comparison to NTC. The use of both angles during the eLAMP assay allows for distinction between high and low initial target concentrations. The efficacy of a smartphone-based platform was also tested and had a similar limit of detection and assay time of less than 10 min. Furthermore, fluorescence-labeled primers were used to validate target nucleic acid amplification. Compared to existing LAMP assays for SARS-CoV-2 detection, these times-to-detections are very rapid. Twit Text FOAVip Emulsion-based isothermal nucleic acid amplification for rapid SARS-CoV-2 detection via angle-dependent light scatter analysis ????Biosens Bioelectron http://www.kidney.de/pget.php?&tw=1&pmid=33640656 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33640656 #FOAvip English Wikipedia <ref>{{Cite pmid|33640656}}</ref> Emulsion-based isothermal nucleic acid amplification for rapid SARS-CoV-2 detection via angle-dependent light scatter analysis #MMPMID33640656 Day AS; Ulep TH; Safavinia B; Hertenstein T; Budiman E; Dieckhaus L; Yoon JY Biosens Bioelectron 2021[May]; 179 (ä): 113099 PMID33640656show ga The SARS-CoV-2 pandemic, an ongoing global health crisis, has revealed the need for new technologies that integrate the sensitivity and specificity of RT-PCR tests with a faster time-to-detection. Here, an emulsion loop-mediated isothermal amplification (eLAMP) platform was developed to allow for the compartmentalization of LAMP reactions, leading to faster changes in emulsion characteristics, and thus lowering time-to-detection. Within these droplets, ongoing LAMP reactions lead to adsorption of amplicons to the water-oil interface, causing a decrease in interfacial tension, resulting in smaller emulsion diameters. Changes in emulsion diameter allow for the monitoring of the reaction by use of angle-dependent light scatter (based off Mie scatter theory). Mie scatter simulations confirmed that light scatter intensity is diameter-dependent and smaller colloids have lower intensity values compared to larger colloids. Via spectrophotometers and fiber optic cables placed at 30 degrees and 60 degrees , light scatter intensity was monitored. Scatter intensities collected at 5 min, 30 degrees could statistically differentiate 10, 10(3), and 10(5) copies/muL initial concentrations compared to NTC. Similarly, 5 min scatter intensities collected at 60 degrees could statistically differentiate 10(5) copies/muL initial concentrations in comparison to NTC. The use of both angles during the eLAMP assay allows for distinction between high and low initial target concentrations. The efficacy of a smartphone-based platform was also tested and had a similar limit of detection and assay time of less than 10 min. Furthermore, fluorescence-labeled primers were used to validate target nucleic acid amplification. Compared to existing LAMP assays for SARS-CoV-2 detection, these times-to-detections are very rapid. |Biosensing Techniques/economics/instrumentation/methods[MESH] |COVID-19 Nucleic Acid Testing/economics/*instrumentation/methods[MESH] |COVID-19/*diagnosis[MESH] |Dynamic Light Scattering/economics/*instrumentation/methods[MESH] |Emulsions/*chemistry[MESH] |Equipment Design[MESH] |Humans[MESH] |Limit of Detection[MESH] |Molecular Diagnostic Techniques/economics/*instrumentation/methods[MESH] |Nucleic Acid Amplification Techniques/economics/*instrumentation/methods[MESH] |SARS-CoV-2/*isolation & purification[MESH] |Smartphone[MESH]Emulsion-based isothermal nucleic acid amplification for rapid SARS-Co(epmc).pdf DeepDyve Pubget Overpricing