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10.1016/j.jviromet.2021.114111

http://scihub22266oqcxt.onion/10.1016/j.jviromet.2021.114111
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33640374!7905383!33640374
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suck abstract from ncbi


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pmid33640374      J+Virol+Methods 2021 ; 291 (ä): 114111
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  • Multiplexed, microscale, microarray-based serological assay for antibodies against all human-relevant coronaviruses #MMPMID33640374
  • Dawson ED; Kuck LR; Blair RH; Taylor AW; Toth E; Knight V; Rowlen KL
  • J Virol Methods 2021[May]; 291 (ä): 114111 PMID33640374show ga
  • Rapid, sensitive, and precise multiplexed assays for serological analysis during candidate COVID-19 vaccine development would streamline clinical trials. The VaxArray Coronavirus (CoV) SeroAssay quantifies IgG antibody binding to 9 pandemic, potentially pandemic, and endemic human CoV spike antigens in 2?h with automated results analysis. IgG antibodies in serum bind to the CoV spike protein capture antigens printed in a microarray format and are labeled with a fluorescent anti-species IgG secondary label. The assay demonstrated excellent lower limits of quantification ranging from 0.3 to 2.0?ng/mL and linear dynamic ranges of 76 to 911-fold. Average precision of 11 % CV and accuracy (% recovery) of 92.5 % over all capture antigens were achieved over 216 replicates representing 3 days and 3 microarray lots. Clinical performance on 263 human serum samples (132 SARS-CoV-2 negatives and 131 positives based on donor-matched RT-PCR and/or date of collection) produced 98.5 % PPA and 100 % NPA.
  • |Antibodies, Viral/*blood[MESH]
  • |Antigens, Viral/immunology[MESH]
  • |COVID-19 Nucleic Acid Testing[MESH]
  • |COVID-19 Testing/methods[MESH]
  • |COVID-19/diagnosis/immunology[MESH]
  • |Coronavirus Infections/*diagnosis/immunology[MESH]
  • |Coronavirus/immunology/*isolation & purification[MESH]
  • |Humans[MESH]
  • |Immunoassay/methods[MESH]
  • |Immunoglobulin G/blood[MESH]
  • |Microarray Analysis/*methods[MESH]
  • |Reproducibility of Results[MESH]
  • |SARS-CoV-2/isolation & purification[MESH]
  • |Sensitivity and Specificity[MESH]
  • |Serologic Tests/*methods[MESH]


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