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10.1016/j.cmi.2021.02.014 http://scihub22266oqcxt.onion/10.1016/j.cmi.2021.02.014 33631334!7898982!33631334     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Clin+Microbiol+Infect 2021 ; 27 (6): 886-891 Nephropedia Template TP {{tp|p=33631334|t=2021. Prolonged SARS-CoV-2 cell culture replication in respiratory samples from patients with severe COVID-19 |pdf=|usr=}}{{33631334}} gab.com Text Prolonged SARS-CoV-2 cell culture replication in respiratory samples from patients with severe COVID-19 JO: Clin Microbiol Infect http://www.kidney.de/pget.php?&tw=1&pmid=33631334 #FOAvip OBJECTIVES: This study compares the infectivity of SARS-CoV-2 in respiratory samples from patients with mild COVID-19 with those from hospitalized patients with severe bilateral pneumonia. In severe COVID-19, we also analysed the presence of neutralizing activity in paired sera. METHODS: We performed cell cultures on 193 real-time reverse transcription polymerase chain reaction respiratory samples, positive for SARS-CoV-2, obtained from 189 patients at various times, from clinical diagnosis to follow-up. Eleven samples were obtained from asymptomatic individuals, 91 samples from 91 outpatients with mild forms of COVID-19 and 91 samples from 87 inpatients with severe pneumonia. In these patients, neutralizing activity was analysed in 30 paired sera collected after symptom onset >10 days. RESULTS: We detected a cytopathic effect (CPE) in 91/193 (47%) samples. Viral viability was maintained for up to 10 days in patients with mild COVID-19. In patients with severe COVID-19, the virus remained viable for up to 32 days after the onset of symptoms. Patients with severe COVID-19 presented infectious virus at a significantly higher rate in the samples with moderate to low viral load (cycle threshold value >/= 26): 32/75 (43%) versus 14/63 (22%) for mild cases (p < 0.01). We observed a positive CPE despite the presence of clear neutralizing activity (NT50 > 1:1024 in 10% (3/30) of samples. DISCUSSION: Patients with severe COVID-19 might shed viable virus during prolonged periods of up to 4 weeks after symptom onset, even when presenting high cycle threshold values in their respiratory samples and despite having developed high neutralizing antibody titres. Twit Text FOAVip Prolonged SARS-CoV-2 cell culture replication in respiratory samples from patients with severe COVID-19 ????Clin Microbiol Infect http://www.kidney.de/pget.php?&tw=1&pmid=33631334 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33631334 #FOAvip English Wikipedia <ref>{{Cite pmid|33631334}}</ref> Prolonged SARS-CoV-2 cell culture replication in respiratory samples from patients with severe COVID-19 #MMPMID33631334 Folgueira MD; Luczkowiak J; Lasala F; Perez-Rivilla A; Delgado R Clin Microbiol Infect 2021[Jun]; 27 (6): 886-891 PMID33631334show ga OBJECTIVES: This study compares the infectivity of SARS-CoV-2 in respiratory samples from patients with mild COVID-19 with those from hospitalized patients with severe bilateral pneumonia. In severe COVID-19, we also analysed the presence of neutralizing activity in paired sera. METHODS: We performed cell cultures on 193 real-time reverse transcription polymerase chain reaction respiratory samples, positive for SARS-CoV-2, obtained from 189 patients at various times, from clinical diagnosis to follow-up. Eleven samples were obtained from asymptomatic individuals, 91 samples from 91 outpatients with mild forms of COVID-19 and 91 samples from 87 inpatients with severe pneumonia. In these patients, neutralizing activity was analysed in 30 paired sera collected after symptom onset >10 days. RESULTS: We detected a cytopathic effect (CPE) in 91/193 (47%) samples. Viral viability was maintained for up to 10 days in patients with mild COVID-19. In patients with severe COVID-19, the virus remained viable for up to 32 days after the onset of symptoms. Patients with severe COVID-19 presented infectious virus at a significantly higher rate in the samples with moderate to low viral load (cycle threshold value >/= 26): 32/75 (43%) versus 14/63 (22%) for mild cases (p < 0.01). We observed a positive CPE despite the presence of clear neutralizing activity (NT50 > 1:1024 in 10% (3/30) of samples. DISCUSSION: Patients with severe COVID-19 might shed viable virus during prolonged periods of up to 4 weeks after symptom onset, even when presenting high cycle threshold values in their respiratory samples and despite having developed high neutralizing antibody titres. |*Virus Cultivation[MESH] |Adult[MESH] |Aged[MESH] |Animals[MESH] |Antibodies, Viral/blood[MESH] |COVID-19 Nucleic Acid Testing[MESH] |COVID-19/*immunology/*virology[MESH] |Cell Culture Techniques[MESH] |Chlorocebus aethiops[MESH] |Cytopathogenic Effect, Viral[MESH] |Female[MESH] |Hospitalization[MESH] |Humans[MESH] |Immunoglobulin G/blood[MESH] |Immunoglobulin M/blood[MESH] |Male[MESH] |Microbial Viability[MESH] |Middle Aged[MESH] |Pneumonia, Viral[MESH] |SARS-CoV-2/*growth & development/pathogenicity[MESH] |Serologic Tests[MESH] |Severity of Illness Index[MESH] |Time Factors[MESH] |Vero Cells[MESH] |Viral Load[MESH] |Virus Shedding[MESH]Prolonged SARS-CoV-2 cell culture replication in respiratory samples f(epmc).pdf DeepDyve Pubget Overpricing