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10.4269/ajtmh.20-1420 http://scihub22266oqcxt.onion/10.4269/ajtmh.20-1420 33630751!8045656!33630751     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Am+J+Trop+Med+Hyg 2021 ; 104 (4): 1513-1515 Nephropedia Template TP {{tp|p=33630751|t=2021. A Low-Cost SARS-CoV-2 rRBD ELISA to Detect Serostatus in Ecuadorian Population with COVID-19 |pdf=|usr=}}{{33630751}} gab.com Text A Low-Cost SARS-CoV-2 rRBD ELISA to Detect Serostatus in Ecuadorian Population with COVID-19 JO: Am J Trop Med Hyg http://www.kidney.de/pget.php?&tw=1&pmid=33630751 #FOAvip Laboratory diagnosis of the COVID-19 relies on RT-PCR to amplify specific fragments of SARS-CoV-2 genome. However, serological tests are required to determine the immune response elicited after infection. Here, we analyzed convalescent sera collected from positive individuals by RT-PCR to SARS-CoV-2 (n = 78), Zika (n = 20), dengue (n = 20), chikungunya (n = 54), intestinal parasites (n = 11), and HIV (n = 1), from different areas of Ecuador, with an in-house ELISA using a SARS-CoV-2 receptor binding domain recombinant (rRBD) antigen to detect IgG antibodies elicited by SARS-CoV-2 infection. Of the 78 samples positive for SARS-CoV-2 by RT-PCR, 73 showed high absorbance value compared with the cutoff and five were negative. All tested sera from other infections showed no reactivity. Sensitivity, specificity, positive predictive value, and negative predictive value were 93.6%, 100%, 100%, and 95.4%, respectively. This in-house anti-IgG rRBD ELISA offers an economic and simple alternative to determine IgG immune responses after SARS-CoV-2 infection. Twit Text FOAVip A Low-Cost SARS-CoV-2 rRBD ELISA to Detect Serostatus in Ecuadorian Population with COVID-19 ????Am J Trop Med Hyg http://www.kidney.de/pget.php?&tw=1&pmid=33630751 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33630751 #FOAvip English Wikipedia <ref>{{Cite pmid|33630751}}</ref> A Low-Cost SARS-CoV-2 rRBD ELISA to Detect Serostatus in Ecuadorian Population with COVID-19 #MMPMID33630751 Guevara A; Vivero S; Nipaz V; Guaraca V; Coloma J Am J Trop Med Hyg 2021[Feb]; 104 (4): 1513-1515 PMID33630751show ga Laboratory diagnosis of the COVID-19 relies on RT-PCR to amplify specific fragments of SARS-CoV-2 genome. However, serological tests are required to determine the immune response elicited after infection. Here, we analyzed convalescent sera collected from positive individuals by RT-PCR to SARS-CoV-2 (n = 78), Zika (n = 20), dengue (n = 20), chikungunya (n = 54), intestinal parasites (n = 11), and HIV (n = 1), from different areas of Ecuador, with an in-house ELISA using a SARS-CoV-2 receptor binding domain recombinant (rRBD) antigen to detect IgG antibodies elicited by SARS-CoV-2 infection. Of the 78 samples positive for SARS-CoV-2 by RT-PCR, 73 showed high absorbance value compared with the cutoff and five were negative. All tested sera from other infections showed no reactivity. Sensitivity, specificity, positive predictive value, and negative predictive value were 93.6%, 100%, 100%, and 95.4%, respectively. This in-house anti-IgG rRBD ELISA offers an economic and simple alternative to determine IgG immune responses after SARS-CoV-2 infection. |Antibodies, Viral/blood[MESH] |COVID-19 Serological Testing/economics/*methods[MESH] |COVID-19/*diagnosis/epidemiology[MESH] |Ecuador/epidemiology[MESH] |Enzyme-Linked Immunosorbent Assay/economics/*methods[MESH] |Humans[MESH] |Immunoglobulin G/blood[MESH] |Protein Binding[MESH] |Protein Domains[MESH] |Recombinant Proteins/immunology[MESH] |SARS-CoV-2/*immunology[MESH]A Low-Cost SARS-CoV-2 rRBD ELISA to Detect Serostatus in Ecuadorian Po(epmc).pdf DeepDyve Pubget Overpricing