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10.1002/jmv.26900 http://scihub22266oqcxt.onion/10.1002/jmv.26900 33629747!8014669!33629747     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Med+Virol 2021 ; 93 (5): 3219-3226 Nephropedia Template TP {{tp|p=33629747|t=2021. Assessment of 12 qualitative RT-PCR commercial kits for the detection of SARS-CoV-2 |pdf=|usr=}}{{33629747}} gab.com Text Assessment of 12 qualitative RT-PCR commercial kits for the detection of SARS-CoV-2 JO: J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=33629747 #FOAvip The emergence of the novel coronavirus, the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in the late months of 2019 had the officials to declare a public health emergency leading to a global response. Public measurements rely on an accurate diagnosis of individuals infected with the virus by using real-time reverse transcriptase-polymerase chain reaction (RT-PCR). The aim of our study is to relate the fundamental clinical and analytical performance of SARS-CoV-2 (RT-PCR) commercial kits. A total of 94 clinical samples were selected. Generally, 400 microl of each respiratory specimen was subjected to extraction using ExiPrep 96 Viral RNA Kit. All kits master mix preparation, cycling protocol, thermocycler, and results interpretation were carried out according to the manufacturer's instructions of use and recommendations. The performance of the kits was comparable except for the LYRA kit as it was less sensitive (F = 67, p < .001). Overall, four kits scored a sensitivity of 100% including: BGI, IQ Real, Sansure, and RADI. For specificity, all the tested kits scored above 95%. The performance of these commercial kits by gene target showed no significant change in CT values which indicates that kits disparities are mainly linked to the oligonucleotide of the gene target. We believe that most of the commercially available RT-PCR kits included in this study can be used for routine diagnosis of patients with SARS-CoV-2. We recommend including kits with multiple targets in order to monitor the virus changes over time. Twit Text FOAVip Assessment of 12 qualitative RT-PCR commercial kits for the detection of SARS-CoV-2 ????J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=33629747 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33629747 #FOAvip English Wikipedia <ref>{{Cite pmid|33629747}}</ref> Assessment of 12 qualitative RT-PCR commercial kits for the detection of SARS-CoV-2 #MMPMID33629747 Altamimi AM; Obeid DA; Alaifan TA; Taha MT; Alhothali MT; Alzahrani FA; Albarrag AM J Med Virol 2021[May]; 93 (5): 3219-3226 PMID33629747show ga The emergence of the novel coronavirus, the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in the late months of 2019 had the officials to declare a public health emergency leading to a global response. Public measurements rely on an accurate diagnosis of individuals infected with the virus by using real-time reverse transcriptase-polymerase chain reaction (RT-PCR). The aim of our study is to relate the fundamental clinical and analytical performance of SARS-CoV-2 (RT-PCR) commercial kits. A total of 94 clinical samples were selected. Generally, 400 microl of each respiratory specimen was subjected to extraction using ExiPrep 96 Viral RNA Kit. All kits master mix preparation, cycling protocol, thermocycler, and results interpretation were carried out according to the manufacturer's instructions of use and recommendations. The performance of the kits was comparable except for the LYRA kit as it was less sensitive (F = 67, p < .001). Overall, four kits scored a sensitivity of 100% including: BGI, IQ Real, Sansure, and RADI. For specificity, all the tested kits scored above 95%. The performance of these commercial kits by gene target showed no significant change in CT values which indicates that kits disparities are mainly linked to the oligonucleotide of the gene target. We believe that most of the commercially available RT-PCR kits included in this study can be used for routine diagnosis of patients with SARS-CoV-2. We recommend including kits with multiple targets in order to monitor the virus changes over time. |*Reagent Kits, Diagnostic/standards[MESH] |*SARS-CoV-2/genetics/isolation & purification[MESH] |COVID-19/*diagnosis/virology[MESH] |Humans[MESH] |Real-Time Polymerase Chain Reaction/*methods[MESH] |Reverse Transcriptase Polymerase Chain Reaction/*methods[MESH]Assessment of 12 qualitative RT-PCR commercial kits for the detection (epmc).pdf DeepDyve Pubget Overpricing