Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1016/j.jpba.2021.113956 http://scihub22266oqcxt.onion/10.1016/j.jpba.2021.113956 33626443!7869607!33626443     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Pharm+Biomed+Anal 2021 ; 197 (ä): 113956 Nephropedia Template TP {{tp|p=33626443|t=2021. A validated UHPLC-MS/MS method for rapid determination of senicapoc in plasma samples |pdf=|usr=}}{{33626443}} gab.com Text A validated UHPLC-MS/MS method for rapid determination of senicapoc in plasma samples JO: J Pharm Biomed Anal http://www.kidney.de/pget.php?&tw=1&pmid=33626443 #FOAvip The clinically tested KCa3.1 channel blocker, senicapoc, has been proven to have excellent pharmacological properties and prior clinical trials found it to be safe for use in patients with sickle cell anaemia. Currently, several preclinical projects are aiming to repurpose senicapoc for other indications, but well-described analytical methods in the literature are lacking. Our aim was to develop a sensitive, rapid and accurate ultra-high-performance liquid chromatography-tandem mass spectrometry method using pneumatically assisted electrospray ionisation (UHPLC-ESI-MS/MS) suitable for the determination of senicapoc in plasma samples. Unfortunately, direct analysis of senicapoc in crude acetonitrile extracts of human plasma samples by UHPLC-ESI-MS/MS was subjected to significant and variable ion suppression from coeluting phospholipids (PLs). The interferences were mainly caused by the presence of phosphatidylcholine and phosphatidylethanolamine classes of PLs, including their lyso-products. However, the PLs were easily removed from crude extracts by filtration through a sorbent with Lewis acid properties which decreased the total ion suppression effect to approximately 5%. Based on this technique, a simple high-throughput UHPLC-MS/MS method was developed and validated for the determination of senicapoc in 100-muL plasma samples. The lower limit of quantification was 0.1?ng/mL. The mean true extraction recovery was close to 100 %. The relative intra-laboratory reproducibility standard deviations of the measured concentrations were 8% and 4% at concentrations of 0.1?ng/mL and 250?ng/mL, respectively. The trueness expressed as the relative bias of the test results was within +/- 2% at concentrations of 1?ng/mL or higher. Twit Text FOAVip A validated UHPLC-MS/MS method for rapid determination of senicapoc in plasma samples ????J Pharm Biomed Anal http://www.kidney.de/pget.php?&tw=1&pmid=33626443 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33626443 #FOAvip English Wikipedia <ref>{{Cite pmid|33626443}}</ref> A validated UHPLC-MS/MS method for rapid determination of senicapoc in plasma samples #MMPMID33626443 Sorensen LK; Petersen A; Granfeldt A; Simonsen U; Hasselstrom JB J Pharm Biomed Anal 2021[Apr]; 197 (ä): 113956 PMID33626443show ga The clinically tested KCa3.1 channel blocker, senicapoc, has been proven to have excellent pharmacological properties and prior clinical trials found it to be safe for use in patients with sickle cell anaemia. Currently, several preclinical projects are aiming to repurpose senicapoc for other indications, but well-described analytical methods in the literature are lacking. Our aim was to develop a sensitive, rapid and accurate ultra-high-performance liquid chromatography-tandem mass spectrometry method using pneumatically assisted electrospray ionisation (UHPLC-ESI-MS/MS) suitable for the determination of senicapoc in plasma samples. Unfortunately, direct analysis of senicapoc in crude acetonitrile extracts of human plasma samples by UHPLC-ESI-MS/MS was subjected to significant and variable ion suppression from coeluting phospholipids (PLs). The interferences were mainly caused by the presence of phosphatidylcholine and phosphatidylethanolamine classes of PLs, including their lyso-products. However, the PLs were easily removed from crude extracts by filtration through a sorbent with Lewis acid properties which decreased the total ion suppression effect to approximately 5%. Based on this technique, a simple high-throughput UHPLC-MS/MS method was developed and validated for the determination of senicapoc in 100-muL plasma samples. The lower limit of quantification was 0.1?ng/mL. The mean true extraction recovery was close to 100 %. The relative intra-laboratory reproducibility standard deviations of the measured concentrations were 8% and 4% at concentrations of 0.1?ng/mL and 250?ng/mL, respectively. The trueness expressed as the relative bias of the test results was within +/- 2% at concentrations of 1?ng/mL or higher. |Acetamides/*blood[MESH] |Animals[MESH] |Chromatography, High Pressure Liquid/*methods[MESH] |Female[MESH] |Filtration/methods[MESH] |Humans[MESH] |Limit of Detection[MESH] |Phospholipids/blood[MESH] |Plasma/*chemistry[MESH] |Reproducibility of Results[MESH] |Spectrometry, Mass, Electrospray Ionization/methods[MESH] |Swine[MESH] |Tandem Mass Spectrometry/*methods[MESH]A validated UHPLC-MS/MS method for rapid determination of senicapoc in(epmc).pdf DeepDyve Pubget Overpricing