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10.1002/pro.4050 http://scihub22266oqcxt.onion/10.1002/pro.4050 33625752!7980513!33625752     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Protein+Sci 2021 ; 30 (4): 728-734 Nephropedia Template TP {{tp|p=33625752|t=2021. Crystallographic molecular replacement using an in silico-generated search model of SARS-CoV-2 ORF8 |pdf=|usr=}}{{33625752}} gab.com Text Crystallographic molecular replacement using an in silico-generated search model of SARS-CoV-2 ORF8 JO: Protein Sci http://www.kidney.de/pget.php?&tw=1&pmid=33625752 #FOAvip The majority of crystal structures are determined by the method of molecular replacement (MR). The range of application of MR is limited mainly by the need for an accurate search model. In most cases, pre-existing experimentally determined structures are used as search models. In favorable cases, ab initio predicted structures have yielded search models adequate for MR. The ORF8 protein of SARS-CoV-2 represents a challenging case for MR using an ab initio prediction because ORF8 has an all beta-sheet fold and few orthologs. We previously determined experimentally the structure of ORF8 using the single anomalous dispersion (SAD) phasing method, having been unable to find an MR solution to the crystallographic phase problem. Following a report of an accurate prediction of the ORF8 structure, we assessed whether the predicted model would have succeeded as an MR search model. A phase problem solution was found, and the resulting structure was refined, yielding structural parameters equivalent to the original experimental solution. Twit Text FOAVip Crystallographic molecular replacement using an in silico-generated search model of SARS-CoV-2 ORF8 ????Protein Sci http://www.kidney.de/pget.php?&tw=1&pmid=33625752 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33625752 #FOAvip English Wikipedia <ref>{{Cite pmid|33625752}}</ref> Crystallographic molecular replacement using an in silico-generated search model of SARS-CoV-2 ORF8 #MMPMID33625752 Flower TG; Hurley JH Protein Sci 2021[Apr]; 30 (4): 728-734 PMID33625752show ga The majority of crystal structures are determined by the method of molecular replacement (MR). The range of application of MR is limited mainly by the need for an accurate search model. In most cases, pre-existing experimentally determined structures are used as search models. In favorable cases, ab initio predicted structures have yielded search models adequate for MR. The ORF8 protein of SARS-CoV-2 represents a challenging case for MR using an ab initio prediction because ORF8 has an all beta-sheet fold and few orthologs. We previously determined experimentally the structure of ORF8 using the single anomalous dispersion (SAD) phasing method, having been unable to find an MR solution to the crystallographic phase problem. Following a report of an accurate prediction of the ORF8 structure, we assessed whether the predicted model would have succeeded as an MR search model. A phase problem solution was found, and the resulting structure was refined, yielding structural parameters equivalent to the original experimental solution. |COVID-19/*virology[MESH] |Crystallography, X-Ray[MESH] |Humans[MESH] |Models, Molecular[MESH] |Protein Conformation[MESH] |SARS-CoV-2/*chemistry[MESH]Crystallographic molecular replacement using an in silico-generated se(epmc).pdf DeepDyve Pubget Overpricing