Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1038/s41598-021-83723-x http://scihub22266oqcxt.onion/10.1038/s41598-021-83723-x 33619321!7900100!33619321     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2021 ; 11 (1): 4310 Nephropedia Template TP {{tp|p=33619321|t=2021. Digital PCR for high sensitivity viral detection in false-negative SARS-CoV-2 patients |pdf=|usr=}}{{33619321}} gab.com Text Digital PCR for high sensitivity viral detection in false-negative SARS-CoV-2 patients JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=33619321 #FOAvip Patients requiring diagnostic testing for coronavirus disease 2019 (COVID-19) are routinely assessed by reverse-transcription quantitative polymerase chain reaction (RT-qPCR) amplification of Sars-CoV-2 virus RNA extracted from oro/nasopharyngeal swabs. Despite the good specificity of the assays certified for SARS-CoV-2 molecular detection, and a theoretical sensitivity of few viral gene copies per reaction, a relatively high rate of false negatives continues to be reported. This is an important challenge in the management of patients on hospital admission and for correct monitoring of the infectivity after the acute phase. In the present report, we show that the use of digital PCR, a high sensitivity method to detect low amplicon numbers, allowed us to correctly detecting infection in swab material in a significant number of false negatives. We show that the implementation of digital PCR methods in the diagnostic assessment of COVID-19 could resolve, at least in part, this timely issue. Twit Text FOAVip Digital PCR for high sensitivity viral detection in false-negative SARS-CoV-2 patients ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=33619321 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33619321 #FOAvip English Wikipedia <ref>{{Cite pmid|33619321}}</ref> Digital PCR for high sensitivity viral detection in false-negative SARS-CoV-2 patients #MMPMID33619321 Poggio P; Songia P; Vavassori C; Ricci V; Banfi C; Barbieri SS; Garoffolo G; Myasoedova VA; Piacentini L; Raucci A; Scopece A; Sommariva E; Vinci MC; Carcione D; Biondi ML; Mancini ME; Formenti A; Andreini D; Assanelli EM; Agostoni P; Camera M; Colombo GI; Pesce M Sci Rep 2021[Feb]; 11 (1): 4310 PMID33619321show ga Patients requiring diagnostic testing for coronavirus disease 2019 (COVID-19) are routinely assessed by reverse-transcription quantitative polymerase chain reaction (RT-qPCR) amplification of Sars-CoV-2 virus RNA extracted from oro/nasopharyngeal swabs. Despite the good specificity of the assays certified for SARS-CoV-2 molecular detection, and a theoretical sensitivity of few viral gene copies per reaction, a relatively high rate of false negatives continues to be reported. This is an important challenge in the management of patients on hospital admission and for correct monitoring of the infectivity after the acute phase. In the present report, we show that the use of digital PCR, a high sensitivity method to detect low amplicon numbers, allowed us to correctly detecting infection in swab material in a significant number of false negatives. We show that the implementation of digital PCR methods in the diagnostic assessment of COVID-19 could resolve, at least in part, this timely issue. |*False Negative Reactions[MESH] |Adult[MESH] |Aged[MESH] |COVID-19/*diagnosis/diagnostic imaging/genetics[MESH] |Diagnostic Tests, Routine/methods[MESH] |Female[MESH] |Humans[MESH] |Male[MESH] |Middle Aged[MESH] |Real-Time Polymerase Chain Reaction/*methods[MESH] |SARS-CoV-2/genetics/*pathogenicity[MESH] |Sensitivity and Specificity[MESH]Digital PCR for high sensitivity viral detection in false-negative SAR(epmc).pdf DeepDyve Pubget Overpricing