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10.1126/scitranslmed.abf2823 http://scihub22266oqcxt.onion/10.1126/scitranslmed.abf2823 33619081!8099176!33619081     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Transl+Med 2021 ; 13 (589): ä Nephropedia Template TP {{tp|p=33619081|t=2021. Lessons from applied large-scale pooling of 133,816 SARS-CoV-2 RT-PCR tests |pdf=|usr=}}{{33619081}} gab.com Text Lessons from applied large-scale pooling of 133,816 SARS-CoV-2 RT-PCR tests JO: Sci Transl Med http://www.kidney.de/pget.php?&tw=1&pmid=33619081 #FOAvip Pooling multiple swab samples before RNA extraction and real-time reverse transcription polymerase chain reaction (RT-PCR) analysis has been proposed as a strategy to reduce costs and increase throughput of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) tests. However, reports on practical large-scale group testing for SARS-CoV-2 have been scant. Key open questions concern reduced sensitivity due to sample dilution, the rate of false positives, the actual efficiency (number of tests saved by pooling), and the impact of infection rate in the population on assay performance. Here, we report an analysis of 133,816 samples collected between April and September 2020 and tested by Dorfman pooling for the presence of SARS-CoV-2. We spared 76% of RNA extraction and RT-PCR tests, despite the frequently changing prevalence (0.5 to 6%). We observed pooling efficiency and sensitivity that exceeded theoretical predictions, which resulted from the nonrandom distribution of positive samples in pools. Overall, our findings support the use of pooling for efficient large-scale SARS-CoV-2 testing. Twit Text FOAVip Lessons from applied large-scale pooling of 133,816 SARS-CoV-2 RT-PCR tests ????Sci Transl Med http://www.kidney.de/pget.php?&tw=1&pmid=33619081 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33619081 #FOAvip English Wikipedia <ref>{{Cite pmid|33619081}}</ref> Lessons from applied large-scale pooling of 133,816 SARS-CoV-2 RT-PCR tests #MMPMID33619081 Barak N; Ben-Ami R; Sido T; Perri A; Shtoyer A; Rivkin M; Licht T; Peretz A; Magenheim J; Fogel I; Livneh A; Daitch Y; Oiknine-Djian E; Benedek G; Dor Y; Wolf DG; Yassour M Sci Transl Med 2021[Apr]; 13 (589): ä PMID33619081show ga Pooling multiple swab samples before RNA extraction and real-time reverse transcription polymerase chain reaction (RT-PCR) analysis has been proposed as a strategy to reduce costs and increase throughput of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) tests. However, reports on practical large-scale group testing for SARS-CoV-2 have been scant. Key open questions concern reduced sensitivity due to sample dilution, the rate of false positives, the actual efficiency (number of tests saved by pooling), and the impact of infection rate in the population on assay performance. Here, we report an analysis of 133,816 samples collected between April and September 2020 and tested by Dorfman pooling for the presence of SARS-CoV-2. We spared 76% of RNA extraction and RT-PCR tests, despite the frequently changing prevalence (0.5 to 6%). We observed pooling efficiency and sensitivity that exceeded theoretical predictions, which resulted from the nonrandom distribution of positive samples in pools. Overall, our findings support the use of pooling for efficient large-scale SARS-CoV-2 testing. |*COVID-19[MESH] |*SARS-CoV-2[MESH] |COVID-19 Testing[MESH] |Humans[MESH] |RNA, Viral/genetics[MESH] |Reverse Transcriptase Polymerase Chain Reaction[MESH] |Sensitivity and Specificity[MESH]Lessons from applied large-scale pooling of 133,816 SARS-CoV-2 RT-PCR (epmc).pdf DeepDyve Pubget Overpricing