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10.1016/j.eimc.2020.12.014 http://scihub22266oqcxt.onion/10.1016/j.eimc.2020.12.014 33618853!7816606!33618853     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Enferm+Infecc+Microbiol+Clin+(Engl+Ed) 2021 ; 40 (8): 428-35 Nephropedia Template TP {{tp|p=33618853|t=2021. Validacion de una tecnica de PCR duplex usando el gen E y RNasa P para el diagnostico de SARS-CoV-2 |pdf=|usr=}}{{33618853}} gab.com Text Validacion de una tecnica de PCR duplex usando el gen E y RNasa P para el diagnostico de SARS-CoV-2 JO: Enferm Infecc Microbiol Clin (Engl Ed) http://www.kidney.de/pget.php?&tw=1&pmid=33618853 #FOAvip INTRODUCTION: Reverse transcriptase - polymerase chain reaction (RT-PCR) is the standard technique for SARS-CoV-2 diagnosis. The World Health Organization recommends the Charite-Berlin protocol for COVID-19 diagnosis, which requires triple PCR, limiting the process capability of laboratories and delaying the results. In order to reduce these limitations, a duplex PCR is validated for the detection of the E and RNase P genes. METHODS: We compared the limit of detection, sensitivity and specificity of the duplex PCR technique (E gene and RNase P) against the monoplex standard (E gene) in RNA samples from a SARS-CoV-2 isolate and 88 clinical specimens with previously known results. The repeatability and reproducibility of the threshold cycle values (Ct) were determined in two independent laboratories of the Faculty of Medicine of the Universidad de Antioquia, using different reagents and real time instruments. RESULTS: There were no significant differences in the Ct results between both techniques (p = 0.84). Using the monoplex PCR of E gene as a reference, the interrater reliability analysis showed similarity between the two techniques, with a kappa coefficient of 0.89, the sensitivity and the specificity of duplex PCR were 90% and 87%, respectively. CONCLUSIONS: Duplex PCR does not affect the sensitivity and specificity reported by the Charite, Berlin protocol, being a useful tool for SARS-CoV-2 screening in clinical samples. Twit Text FOAVip Validacion de una tecnica de PCR duplex usando el gen E y RNasa P para el diagnostico de SARS-CoV-2 ????Enferm Infecc Microbiol Clin (Engl Ed) http://www.kidney.de/pget.php?&tw=1&pmid=33618853 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33618853 #FOAvip English Wikipedia <ref>{{Cite pmid|33618853}}</ref> Validacion de una tecnica de PCR duplex usando el gen E y RNasa P para el diagnostico de SARS-CoV-2 #MMPMID33618853 Palacio Rua K; Garcia Correa JF; Aguilar-Jimenez W; Afanador Ayala C; Rugeles MT; Zuluaga AF Enferm Infecc Microbiol Clin (Engl Ed) 2021[Jan]; 40 (8): 428-35 PMID33618853show ga INTRODUCTION: Reverse transcriptase - polymerase chain reaction (RT-PCR) is the standard technique for SARS-CoV-2 diagnosis. The World Health Organization recommends the Charite-Berlin protocol for COVID-19 diagnosis, which requires triple PCR, limiting the process capability of laboratories and delaying the results. In order to reduce these limitations, a duplex PCR is validated for the detection of the E and RNase P genes. METHODS: We compared the limit of detection, sensitivity and specificity of the duplex PCR technique (E gene and RNase P) against the monoplex standard (E gene) in RNA samples from a SARS-CoV-2 isolate and 88 clinical specimens with previously known results. The repeatability and reproducibility of the threshold cycle values (Ct) were determined in two independent laboratories of the Faculty of Medicine of the Universidad de Antioquia, using different reagents and real time instruments. RESULTS: There were no significant differences in the Ct results between both techniques (p = 0.84). Using the monoplex PCR of E gene as a reference, the interrater reliability analysis showed similarity between the two techniques, with a kappa coefficient of 0.89, the sensitivity and the specificity of duplex PCR were 90% and 87%, respectively. CONCLUSIONS: Duplex PCR does not affect the sensitivity and specificity reported by the Charite, Berlin protocol, being a useful tool for SARS-CoV-2 screening in clinical samples. äValidacion de una tecnica de PCR duplex usando el gen E y RNasa P para(epmc).pdf DeepDyve Pubget Overpricing