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10.1186/s13073-021-00847-5 http://scihub22266oqcxt.onion/10.1186/s13073-021-00847-5 33618765!7898256!33618765     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Genome+Med 2021 ; 13 (1): 30 Nephropedia Template TP {{tp|p=33618765|t=2021. Intra-host variation and evolutionary dynamics of SARS-CoV-2 populations in COVID-19 patients |pdf=|usr=}}{{33618765}} gab.com Text Intra-host variation and evolutionary dynamics of SARS-CoV-2 populations in COVID-19 patients JO: Genome Med http://www.kidney.de/pget.php?&tw=1&pmid=33618765 #FOAvip BACKGROUND: Since early February 2021, the causative agent of COVID-19, SARS-CoV-2, has infected over 104 million people with more than 2 million deaths according to official reports. The key to understanding the biology and virus-host interactions of SARS-CoV-2 requires the knowledge of mutation and evolution of this virus at both inter- and intra-host levels. However, despite quite a few polymorphic sites identified among SARS-CoV-2 populations, intra-host variant spectra and their evolutionary dynamics remain mostly unknown. METHODS: Using high-throughput sequencing of metatranscriptomic and hybrid captured libraries, we characterized consensus genomes and intra-host single nucleotide variations (iSNVs) of serial samples collected from eight patients with COVID-19. The distribution of iSNVs along the SARS-CoV-2 genome was analyzed and co-occurring iSNVs among COVID-19 patients were identified. We also compared the evolutionary dynamics of SARS-CoV-2 population in the respiratory tract (RT) and gastrointestinal tract (GIT). RESULTS: The 32 consensus genomes revealed the co-existence of different genotypes within the same patient. We further identified 40 intra-host single nucleotide variants (iSNVs). Most (30/40) iSNVs presented in a single patient, while ten iSNVs were found in at least two patients or identical to consensus variants. Comparing allele frequencies of the iSNVs revealed a clear genetic differentiation between intra-host populations from the respiratory tract (RT) and gastrointestinal tract (GIT), mostly driven by bottleneck events during intra-host migrations. Compared to RT populations, the GIT populations showed a better maintenance and rapid development of viral genetic diversity following the suspected intra-host bottlenecks. CONCLUSIONS: Our findings here illustrate the intra-host bottlenecks and evolutionary dynamics of SARS-CoV-2 in different anatomic sites and may provide new insights to understand the virus-host interactions of coronaviruses and other RNA viruses. Twit Text FOAVip Intra-host variation and evolutionary dynamics of SARS-CoV-2 populations in COVID-19 patients ????Genome Med http://www.kidney.de/pget.php?&tw=1&pmid=33618765 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33618765 #FOAvip English Wikipedia <ref>{{Cite pmid|33618765}}</ref> Intra-host variation and evolutionary dynamics of SARS-CoV-2 populations in COVID-19 patients #MMPMID33618765 Wang Y; Wang D; Zhang L; Sun W; Zhang Z; Chen W; Zhu A; Huang Y; Xiao F; Yao J; Gan M; Li F; Luo L; Huang X; Zhang Y; Wong SS; Cheng X; Ji J; Ou Z; Xiao M; Li M; Li J; Ren P; Deng Z; Zhong H; Xu X; Song T; Mok CKP; Peiris M; Zhong N; Zhao J; Li Y; Li J; Zhao J Genome Med 2021[Feb]; 13 (1): 30 PMID33618765show ga BACKGROUND: Since early February 2021, the causative agent of COVID-19, SARS-CoV-2, has infected over 104 million people with more than 2 million deaths according to official reports. The key to understanding the biology and virus-host interactions of SARS-CoV-2 requires the knowledge of mutation and evolution of this virus at both inter- and intra-host levels. However, despite quite a few polymorphic sites identified among SARS-CoV-2 populations, intra-host variant spectra and their evolutionary dynamics remain mostly unknown. METHODS: Using high-throughput sequencing of metatranscriptomic and hybrid captured libraries, we characterized consensus genomes and intra-host single nucleotide variations (iSNVs) of serial samples collected from eight patients with COVID-19. The distribution of iSNVs along the SARS-CoV-2 genome was analyzed and co-occurring iSNVs among COVID-19 patients were identified. We also compared the evolutionary dynamics of SARS-CoV-2 population in the respiratory tract (RT) and gastrointestinal tract (GIT). RESULTS: The 32 consensus genomes revealed the co-existence of different genotypes within the same patient. We further identified 40 intra-host single nucleotide variants (iSNVs). Most (30/40) iSNVs presented in a single patient, while ten iSNVs were found in at least two patients or identical to consensus variants. Comparing allele frequencies of the iSNVs revealed a clear genetic differentiation between intra-host populations from the respiratory tract (RT) and gastrointestinal tract (GIT), mostly driven by bottleneck events during intra-host migrations. Compared to RT populations, the GIT populations showed a better maintenance and rapid development of viral genetic diversity following the suspected intra-host bottlenecks. CONCLUSIONS: Our findings here illustrate the intra-host bottlenecks and evolutionary dynamics of SARS-CoV-2 in different anatomic sites and may provide new insights to understand the virus-host interactions of coronaviruses and other RNA viruses. |*Polymorphism, Single Nucleotide[MESH] |COVID-19/*prevention & control/virology[MESH] |Gene Frequency[MESH] |Genome, Viral/*genetics[MESH] |Genotype[MESH] |Haplotypes[MESH] |High-Throughput Nucleotide Sequencing/*methods[MESH] |Host-Pathogen Interactions[MESH] |Humans[MESH] |Phylogeny[MESH]Intra-host variation and evolutionary dynamics of SARS-CoV-2 populatio(epmc).pdf DeepDyve Pubget Overpricing