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10.7754/Clin.Lab.2020.200731 http://scihub22266oqcxt.onion/10.7754/Clin.Lab.2020.200731 33616332!ä!33616332 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Clin+Lab 2021 ; 67 (2): ä Nephropedia Template TP {{tp|p=33616332|t=2021. Unmatched Virus Preservation Solution may Impede One-Step Reagent for Detection of 2019-nCoV RNA |pdf=|usr=}}{{33616332}} gab.com Text Unmatched Virus Preservation Solution may Impede One-Step Reagent for Detection of 2019-nCoV RNA JO: Clin Lab http://www.kidney.de/pget.php?&tw=1&pmid=33616332 #FOAvip BACKGROUND AND METHODS: 2019 Corona Virus Disease (COVID-19) caused by SARS-CoV-2 is still pandemic now. RT-qPCR detection was the most common method for the diagnosis of SARS-CoV-2 infection, facilitated by amounts of nucleic acid testing kits. However, the accuracy of nucleic acid detection is affected by various factors such as specimen collection, specimen preparation, reagents deficiency, and personnel quality. RESULTS: In this study, we found that unmatched virus preservation solution will inhibit N gene and OFR-1ab gene (two independent genes of SARS-CoV-2) amplification in one-step detection reagent. CONCLUSIONS: Despite just being a particular phenomenon we found in our work to fight 2019-nCoV, we concluded that unmatched virus preservation solution may have an inhibitory effect on SARS-CoV-2 nucleic acid detection which may lead to incorrect clinical diagnosis. Twit Text FOAVip Unmatched Virus Preservation Solution may Impede One-Step Reagent for Detection of 2019-nCoV RNA ????Clin Lab http://www.kidney.de/pget.php?&tw=1&pmid=33616332 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33616332 #FOAvip English Wikipedia <ref>{{Cite pmid|33616332}}</ref> Unmatched Virus Preservation Solution may Impede One-Step Reagent for Detection of 2019-nCoV RNA #MMPMID33616332 Liu W; Guo X; Deng J; Song Y; Yin M; Zhang C; Long X; Cao C Clin Lab 2021[Feb]; 67 (2): ä PMID33616332show ga BACKGROUND AND METHODS: 2019 Corona Virus Disease (COVID-19) caused by SARS-CoV-2 is still pandemic now. RT-qPCR detection was the most common method for the diagnosis of SARS-CoV-2 infection, facilitated by amounts of nucleic acid testing kits. However, the accuracy of nucleic acid detection is affected by various factors such as specimen collection, specimen preparation, reagents deficiency, and personnel quality. RESULTS: In this study, we found that unmatched virus preservation solution will inhibit N gene and OFR-1ab gene (two independent genes of SARS-CoV-2) amplification in one-step detection reagent. CONCLUSIONS: Despite just being a particular phenomenon we found in our work to fight 2019-nCoV, we concluded that unmatched virus preservation solution may have an inhibitory effect on SARS-CoV-2 nucleic acid detection which may lead to incorrect clinical diagnosis. |*COVID-19/diagnosis/virology[MESH] |*SARS-CoV-2/genetics/isolation & purification[MESH] |*Specimen Handling/adverse effects/methods[MESH] |COVID-19 Nucleic Acid Testing/*methods[MESH] |Diagnostic Errors/prevention & control[MESH] |Genes, Viral/*drug effects[MESH] |Humans[MESH] |Organ Preservation Solutions/*pharmacology[MESH]Unmatched Virus Preservation Solution may Impede One-Step Reagent for (epmc).pdf DeepDyve Pubget Overpricing