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10.1002/bit.27718 http://scihub22266oqcxt.onion/10.1002/bit.27718 33615437!8013395!33615437     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biotechnol+Bioeng 2021 ; 118 (5): 2053-2066 Nephropedia Template TP {{tp|p=33615437|t=2021. A CRISPR/Cas9 eraser strategy for contamination-free PCR end-point detection |pdf=|usr=}}{{33615437}} gab.com Text A CRISPR/Cas9 eraser strategy for contamination-free PCR end-point detection JO: Biotechnol Bioeng http://www.kidney.de/pget.php?&tw=1&pmid=33615437 #FOAvip Polymerase chain reaction (PCR), a central technology for molecular diagnostics, is highly sensitive but susceptible to the risk of false positives caused by aerosol contamination, especially when an end-point detection mode is applied. Here, we proposed a solution by designing a clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 eraser strategy for eliminating potential contamination amplification. The CRISPR/Cas9 engineered eraser is firstly adopted into artpcr reverse-transcription PCR (RT-PCR) system to achieve contamination-free RNA detection. Subsequently, we extended this CRISPR/Cas9 eraser to the PCR system. We engineered conventional PCR primers to enable the amplified products to contain an implanted NGG (protospacer adjacent motif, PAM) site, which is used as a code for specific CRISPR/Cas9 recognition. Pre-incubation of Cas9/sgRNA with PCR mix leads to a selective cleavage of contamination amplicons, thus only the template DNA is amplified. The developed CRISPR/Cas9 eraser, adopted by both RT-PCR and PCR systems, showed high-fidelity detection of SARS-CoV-2 and African swine fever virus with a convenient strip test. Twit Text FOAVip A CRISPR/Cas9 eraser strategy for contamination-free PCR end-point detection ????Biotechnol Bioeng http://www.kidney.de/pget.php?&tw=1&pmid=33615437 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33615437 #FOAvip English Wikipedia <ref>{{Cite pmid|33615437}}</ref> A CRISPR/Cas9 eraser strategy for contamination-free PCR end-point detection #MMPMID33615437 Lin W; Tian T; Jiang Y; Xiong E; Zhu D; Zhou X Biotechnol Bioeng 2021[May]; 118 (5): 2053-2066 PMID33615437show ga Polymerase chain reaction (PCR), a central technology for molecular diagnostics, is highly sensitive but susceptible to the risk of false positives caused by aerosol contamination, especially when an end-point detection mode is applied. Here, we proposed a solution by designing a clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 eraser strategy for eliminating potential contamination amplification. The CRISPR/Cas9 engineered eraser is firstly adopted into artpcr reverse-transcription PCR (RT-PCR) system to achieve contamination-free RNA detection. Subsequently, we extended this CRISPR/Cas9 eraser to the PCR system. We engineered conventional PCR primers to enable the amplified products to contain an implanted NGG (protospacer adjacent motif, PAM) site, which is used as a code for specific CRISPR/Cas9 recognition. Pre-incubation of Cas9/sgRNA with PCR mix leads to a selective cleavage of contamination amplicons, thus only the template DNA is amplified. The developed CRISPR/Cas9 eraser, adopted by both RT-PCR and PCR systems, showed high-fidelity detection of SARS-CoV-2 and African swine fever virus with a convenient strip test. |*CRISPR-Cas Systems[MESH] |African Swine Fever Virus/isolation & purification[MESH] |Animals[MESH] |Humans[MESH] |Polymerase Chain Reaction/*methods[MESH] |RNA, Guide, CRISPR-Cas Systems[MESH] |RNA, Viral/isolation & purification[MESH] |Reverse Transcriptase Polymerase Chain Reaction/*methods[MESH] |SARS-CoV-2/isolation & purification[MESH]A CRISPR/Cas9 eraser strategy for contamination-free PCR end-point det(epmc).pdf DeepDyve Pubget Overpricing