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10.3762/bjnano.12.13 http://scihub22266oqcxt.onion/10.3762/bjnano.12.13 33614383!7871036!33614383     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Beilstein+J+Nanotechnol 2021 ; 12 (ä): 172-179 Nephropedia Template TP {{tp|p=33614383|t=2021. Imaging of SARS-CoV-2 infected Vero E6 cells by helium ion microscopy |pdf=|usr=}}{{33614383}} gab.com Text Imaging of SARS-CoV-2 infected Vero E6 cells by helium ion microscopy JO: Beilstein J Nanotechnol http://www.kidney.de/pget.php?&tw=1&pmid=33614383 #FOAvip Helium ion microscopy (HIM) offers the opportunity to obtain direct views of biological samples such as cellular structures, virus particles, and microbial interactions. Imaging with the HIM combines sub-nanometer resolution, large depth of field, and high surface sensitivity. Due to its charge compensation capability, the HIM can image insulating biological samples without additional conductive coatings. Here, we present an exploratory HIM study of SARS-CoV-2 infected Vero E6 cells, in which several areas of interaction between cells and virus particles, as well as among virus particles, were imaged. The HIM pictures show the three-dimensional appearance of SARS-CoV-2 and the surface of Vero E6 cells at a multiplicity of infection of approximately 1 with great morphological detail. The absence of a conductive coating allows for a distinction between virus particles bound to the cell membrane and virus particles lying on top of the membrane. After prolonged imaging, it was found that ion-induced deposition of hydrocarbons from the vacuum renders the sample sufficiently conductive to allow for imaging even without charge compensation. The presented images demonstrate the potential of the HIM in bioimaging, especially for the imaging of interactions between viruses and their host organisms. Twit Text FOAVip Imaging of SARS-CoV-2 infected Vero E6 cells by helium ion microscopy ????Beilstein J Nanotechnol http://www.kidney.de/pget.php?&tw=1&pmid=33614383 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33614383 #FOAvip English Wikipedia <ref>{{Cite pmid|33614383}}</ref> Imaging of SARS-CoV-2 infected Vero E6 cells by helium ion microscopy #MMPMID33614383 Frese N; Schmerer P; Wortmann M; Schurmann M; Konig M; Westphal M; Weber F; Sudhoff H; Golzhauser A Beilstein J Nanotechnol 2021[]; 12 (ä): 172-179 PMID33614383show ga Helium ion microscopy (HIM) offers the opportunity to obtain direct views of biological samples such as cellular structures, virus particles, and microbial interactions. Imaging with the HIM combines sub-nanometer resolution, large depth of field, and high surface sensitivity. Due to its charge compensation capability, the HIM can image insulating biological samples without additional conductive coatings. Here, we present an exploratory HIM study of SARS-CoV-2 infected Vero E6 cells, in which several areas of interaction between cells and virus particles, as well as among virus particles, were imaged. The HIM pictures show the three-dimensional appearance of SARS-CoV-2 and the surface of Vero E6 cells at a multiplicity of infection of approximately 1 with great morphological detail. The absence of a conductive coating allows for a distinction between virus particles bound to the cell membrane and virus particles lying on top of the membrane. After prolonged imaging, it was found that ion-induced deposition of hydrocarbons from the vacuum renders the sample sufficiently conductive to allow for imaging even without charge compensation. The presented images demonstrate the potential of the HIM in bioimaging, especially for the imaging of interactions between viruses and their host organisms. äImaging of SARS-CoV-2 infected Vero E6 cells by helium ion microscopy (epmc).pdf DeepDyve Pubget Overpricing