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10.1093/pubmed/fdab011

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33611565!7928805!33611565
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suck abstract from ncbi


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pmid33611565      J+Public+Health+(Oxf) 2022 ; 44 (2): e260-e263
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  • The use of dried blood spots for the serological evaluation of SARS-CoV-2 antibodies #MMPMID33611565
  • Toh ZQ; Higgins RA; Anderson J; Mazarakis N; Do LAH; Rautenbacher K; Ramos P; Dohle K; Tosif S; Crawford N; Mulholland K; Licciardi PV
  • J Public Health (Oxf) 2022[Jun]; 44 (2): e260-e263 PMID33611565show ga
  • BACKGROUND: To determine if dried blood spot specimens (DBS) can reliably detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies, we compared the SARS-CoV-2 IgG antibody response in paired serum and eluates from DBS specimens. METHODS: A total of 95 paired DBS and serum samples were collected from 74 participants (aged 1-63 years) as part of a household cohort study in Melbourne, Australia. SARS-CoV-2 IgG antibodies specific for the receptor-binding domain (RBD) and S1 proteins between serum and eluates from DBS specimens were compared using an FDA-approved ELISA method. RESULTS: Among the 74 participants, 42% (31/74) were children and the rest were adults. A total of 16 children and 13 adults were SARS-CoV-2 positive by polymerase chain reaction. The IgG seropositivity rate was similar between serum and DBS specimens (18.9% (18/95) versus 16.8% (16/95)), respectively. Similar RBD and S1-specific IgG levels were detected between serum and DBS specimens. Serum IgG levels strongly correlated with DBS IgG levels (r = 0.99, P < 0.0001) for both SARS-CoV-2 proteins. Furthermore, antibodies remained stable in DBS specimens for >3 months. CONCLUSIONS: DBS specimens can be reliably used as an alternative to serum samples for SARS-CoV-2 antibody measurement. The use of DBS specimens would facilitate serosurveillance efforts particularly in hard-to-reach populations and inform public health responses including COVID-19 vaccination strategies.
  • |*COVID-19/diagnosis[MESH]
  • |*SARS-CoV-2[MESH]
  • |Adult[MESH]
  • |Antibodies, Viral[MESH]
  • |COVID-19 Vaccines[MESH]
  • |Child[MESH]
  • |Cohort Studies[MESH]
  • |Humans[MESH]


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