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10.1002/jcla.23735 http://scihub22266oqcxt.onion/10.1002/jcla.23735 33608968!7995104!33608968     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Clin+Lab+Anal 2021 ; 35 (4): e23735 Nephropedia Template TP {{tp|p=33608968|t=2021. Large scale production and characterization of SARS-CoV-2 whole antigen for serological test development |pdf=|usr=}}{{33608968}} gab.com Text Large scale production and characterization of SARS-CoV-2 whole antigen for serological test development JO: J Clin Lab Anal http://www.kidney.de/pget.php?&tw=1&pmid=33608968 #FOAvip BACKGROUND: The rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has generated a pandemic with alarming rates of fatality worldwide. This situation has had a major impact on clinical laboratories that have attempted to answer the urgent need for diagnostic tools, since the identification of coronavirus disease 2019 (COVID-19). Development of a reliable serological diagnostic immunoassay, with high levels of sensitivity and specificity to detect SARS-CoV-2 antibodies with improved differential diagnosis from other circulating viruses, is mandatory. METHODS: An enzyme-linked immunosorbent assay (ELISA) using whole inactivated virus cultured in vitro, was developed to detect viral antigens. WB and ELISA investigations were carried out with sera of convalescent patients and negative sera samples. Both analyses were concurrently performed with recombinant MABs to verify the findings. RESULTS: Preliminary data from 10 sera (5 patients with COVID-19, and 5 healthy controls) using this immunoassay are very promising, successfully identifying all of the confirmed SARS-CoV-2-positive individuals. CONCLUSION: This ELISA appears to be a specific and reliable method for detecting COVID-19 antibodies (IgG, IgM, and IgA), and a useful tool for identifying individuals which have developed immunity to the virus. Twit Text FOAVip Large scale production and characterization of SARS-CoV-2 whole antigen for serological test development ????J Clin Lab Anal http://www.kidney.de/pget.php?&tw=1&pmid=33608968 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33608968 #FOAvip English Wikipedia <ref>{{Cite pmid|33608968}}</ref> Large scale production and characterization of SARS-CoV-2 whole antigen for serological test development #MMPMID33608968 Cerutti H; Ricci V; Tesi G; Soldatini C; Castria M; Vaccaro MN; Tornesi S; Toppi S; Verdiani S; Brogi A J Clin Lab Anal 2021[Apr]; 35 (4): e23735 PMID33608968show ga BACKGROUND: The rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has generated a pandemic with alarming rates of fatality worldwide. This situation has had a major impact on clinical laboratories that have attempted to answer the urgent need for diagnostic tools, since the identification of coronavirus disease 2019 (COVID-19). Development of a reliable serological diagnostic immunoassay, with high levels of sensitivity and specificity to detect SARS-CoV-2 antibodies with improved differential diagnosis from other circulating viruses, is mandatory. METHODS: An enzyme-linked immunosorbent assay (ELISA) using whole inactivated virus cultured in vitro, was developed to detect viral antigens. WB and ELISA investigations were carried out with sera of convalescent patients and negative sera samples. Both analyses were concurrently performed with recombinant MABs to verify the findings. RESULTS: Preliminary data from 10 sera (5 patients with COVID-19, and 5 healthy controls) using this immunoassay are very promising, successfully identifying all of the confirmed SARS-CoV-2-positive individuals. CONCLUSION: This ELISA appears to be a specific and reliable method for detecting COVID-19 antibodies (IgG, IgM, and IgA), and a useful tool for identifying individuals which have developed immunity to the virus. |*Antigens, Viral/chemistry/immunology/isolation & purification[MESH] |*SARS-CoV-2/chemistry/immunology/isolation & purification[MESH] |Animals[MESH] |Antibodies, Viral/blood[MESH] |Blotting, Western[MESH] |COVID-19 Serological Testing/*methods[MESH] |COVID-19/*diagnosis/immunology/virology[MESH] |Chlorocebus aethiops[MESH] |Coronavirus Nucleocapsid Proteins/chemistry/immunology/isolation & purification[MESH] |Enzyme-Linked Immunosorbent Assay/methods[MESH] |Humans[MESH] |Phosphoproteins/chemistry/immunology/isolation & purification[MESH] |Spike Glycoprotein, Coronavirus/chemistry/immunology/isolation & purification[MESH] |Vero Cells[MESH]Large scale production and characterization of SARS-CoV-2 whole antige(epmc).pdf DeepDyve Pubget Overpricing