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10.1016/j.bjid.2021.101543 http://scihub22266oqcxt.onion/10.1016/j.bjid.2021.101543 33607081!7883731!33607081     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Braz+J+Infect+Dis 2021 ; 25 (2): 101543 Nephropedia Template TP {{tp|p=33607081|t=2021. Validation of the GeneXpert Xpress SARS-CoV-2 PCR assay using saliva as biological specimen |pdf=|usr=}}{{33607081}} gab.com Text Validation of the GeneXpert Xpress SARS-CoV-2 PCR assay using saliva as biological specimen JO: Braz J Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=33607081 #FOAvip In the pandemic, rapid and accurate detection of SARS-CoV-2 is crucial in controlling the outbreak. Recent studies have shown a high detection rate using saliva/oral fluids as specimens for laboratory detection of the virus. We intended to evaluate the test performance of the Xpert Xpress SARS-CoV-2 cartridge assay in comparison to a conventional qRT-PCR testing, using saliva as biological specimen. Forty saliva samples from symptomatic participants were collected. Conventional qRT-PCR was performed for amplification of E and RdRp genes and the Xpert Xpress SARS-CoV-2 assay amplified E and N2 genes. In the conventional assay, the median cycle threshold value of the E gene was 34.9, and of the RdRp gene was 38.3. In the Xpert Xpress assay, the median cycle threshold value of the E gene was 29.7, and of the N2 gene was 31.6. These results can allow a broaden use of molecular tests for management of COVID-19 pandemic, especially in resources-limited settings. Twit Text FOAVip Validation of the GeneXpert Xpress SARS-CoV-2 PCR assay using saliva as biological specimen ????Braz J Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=33607081 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33607081 #FOAvip English Wikipedia <ref>{{Cite pmid|33607081}}</ref> Validation of the GeneXpert Xpress SARS-CoV-2 PCR assay using saliva as biological specimen #MMPMID33607081 Vaz SN; Santana DS; Netto EM; Wang WK; Brites C Braz J Infect Dis 2021[Mar]; 25 (2): 101543 PMID33607081show ga In the pandemic, rapid and accurate detection of SARS-CoV-2 is crucial in controlling the outbreak. Recent studies have shown a high detection rate using saliva/oral fluids as specimens for laboratory detection of the virus. We intended to evaluate the test performance of the Xpert Xpress SARS-CoV-2 cartridge assay in comparison to a conventional qRT-PCR testing, using saliva as biological specimen. Forty saliva samples from symptomatic participants were collected. Conventional qRT-PCR was performed for amplification of E and RdRp genes and the Xpert Xpress SARS-CoV-2 assay amplified E and N2 genes. In the conventional assay, the median cycle threshold value of the E gene was 34.9, and of the RdRp gene was 38.3. In the Xpert Xpress assay, the median cycle threshold value of the E gene was 29.7, and of the N2 gene was 31.6. These results can allow a broaden use of molecular tests for management of COVID-19 pandemic, especially in resources-limited settings. |*COVID-19[MESH] |*SARS-CoV-2[MESH] |COVID-19 Testing[MESH] |Clinical Laboratory Techniques[MESH] |Humans[MESH] |Nasopharynx[MESH] |Pandemics[MESH] |Polymerase Chain Reaction[MESH] |Saliva[MESH] |Sensitivity and Specificity[MESH]Validation of the GeneXpert Xpress SARS-CoV-2 PCR assay using saliva a(epmc).pdf DeepDyve Pubget Overpricing