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10.3201/eid2704.204337 http://scihub22266oqcxt.onion/10.3201/eid2704.204337 33600302!8007326!33600302     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Emerg+Infect+Dis 2021 ; 27 (4): 1023-1031 Nephropedia Template TP {{tp|p=33600302|t=2021. Difficulties in Differentiating Coronaviruses from Subcellular Structures in Human Tissues by Electron Microscopy |pdf=|usr=}}{{33600302}} gab.com Text Difficulties in Differentiating Coronaviruses from Subcellular Structures in Human Tissues by Electron Microscopy JO: Emerg Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=33600302 #FOAvip Efforts to combat the coronavirus disease (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have placed a renewed focus on the use of transmission electron microscopy for identifying coronavirus in tissues. In attempts to attribute pathology of COVID-19 patients directly to tissue damage caused by SARS-CoV-2, investigators have inaccurately reported subcellular structures, including coated vesicles, multivesicular bodies, and vesiculating rough endoplasmic reticulum, as coronavirus particles. We describe morphologic features of coronavirus that distinguish it from subcellular structures, including particle size range (60-140 nm), intracellular particle location within membrane-bound vacuoles, and a nucleocapsid appearing in cross section as dense dots (6-12 nm) within the particles. In addition, although the characteristic spikes of coronaviruses may be visible on the virus surface, especially on extracellular particles, they are less evident in thin sections than in negative stain preparations. Twit Text FOAVip Difficulties in Differentiating Coronaviruses from Subcellular Structures in Human Tissues by Electron Microscopy ????Emerg Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=33600302 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33600302 #FOAvip English Wikipedia <ref>{{Cite pmid|33600302}}</ref> Difficulties in Differentiating Coronaviruses from Subcellular Structures in Human Tissues by Electron Microscopy #MMPMID33600302 Bullock HA; Goldsmith CS; Zaki SR; Martines RB; Miller SE Emerg Infect Dis 2021[Apr]; 27 (4): 1023-1031 PMID33600302show ga Efforts to combat the coronavirus disease (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have placed a renewed focus on the use of transmission electron microscopy for identifying coronavirus in tissues. In attempts to attribute pathology of COVID-19 patients directly to tissue damage caused by SARS-CoV-2, investigators have inaccurately reported subcellular structures, including coated vesicles, multivesicular bodies, and vesiculating rough endoplasmic reticulum, as coronavirus particles. We describe morphologic features of coronavirus that distinguish it from subcellular structures, including particle size range (60-140 nm), intracellular particle location within membrane-bound vacuoles, and a nucleocapsid appearing in cross section as dense dots (6-12 nm) within the particles. In addition, although the characteristic spikes of coronaviruses may be visible on the virus surface, especially on extracellular particles, they are less evident in thin sections than in negative stain preparations. |*COVID-19/pathology/virology[MESH] |*Cellular Structures/classification/ultrastructure[MESH] |*SARS-CoV-2/isolation & purification/ultrastructure[MESH] |Biopsy/methods[MESH] |Humans[MESH]Difficulties in Differentiating Coronaviruses from Subcellular Structu(epmc).pdf DeepDyve Pubget Overpricing