Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1126/sciadv.abf1738 http://scihub22266oqcxt.onion/10.1126/sciadv.abf1738 33597251!7888922!33597251     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Adv 2021 ; 7 (8): ä Nephropedia Template TP {{tp|p=33597251|t=2021. An engineered decoy receptor for SARS-CoV-2 broadly binds protein S sequence variants |pdf=|usr=}}{{33597251}} gab.com Text An engineered decoy receptor for SARS-CoV-2 broadly binds protein S sequence variants JO: Sci Adv http://www.kidney.de/pget.php?&tw=1&pmid=33597251 #FOAvip The spike S of SARS-CoV-2 recognizes ACE2 on the host cell membrane to initiate entry. Soluble decoy receptors, in which the ACE2 ectodomain is engineered to block S with high affinity, potently neutralize infection and, because of close similarity with the natural receptor, hold out the promise of being broadly active against virus variants without opportunity for escape. Here, we directly test this hypothesis. We find that an engineered decoy receptor, sACE2(2)v2.4, tightly binds S of SARS-associated viruses from humans and bats, despite the ACE2-binding surface being a region of high diversity. Saturation mutagenesis of the receptor-binding domain followed by in vitro selection, with wild-type ACE2 and the engineered decoy competing for binding sites, failed to find S mutants that discriminate in favor of the wild-type receptor. We conclude that resistance to engineered decoys will be rare and that decoys may be active against future outbreaks of SARS-associated betacoronaviruses. Twit Text FOAVip An engineered decoy receptor for SARS-CoV-2 broadly binds protein S sequence variants ????Sci Adv http://www.kidney.de/pget.php?&tw=1&pmid=33597251 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33597251 #FOAvip English Wikipedia <ref>{{Cite pmid|33597251}}</ref> An engineered decoy receptor for SARS-CoV-2 broadly binds protein S sequence variants #MMPMID33597251 Chan KK; Tan TJC; Narayanan KK; Procko E Sci Adv 2021[Feb]; 7 (8): ä PMID33597251show ga The spike S of SARS-CoV-2 recognizes ACE2 on the host cell membrane to initiate entry. Soluble decoy receptors, in which the ACE2 ectodomain is engineered to block S with high affinity, potently neutralize infection and, because of close similarity with the natural receptor, hold out the promise of being broadly active against virus variants without opportunity for escape. Here, we directly test this hypothesis. We find that an engineered decoy receptor, sACE2(2)v2.4, tightly binds S of SARS-associated viruses from humans and bats, despite the ACE2-binding surface being a region of high diversity. Saturation mutagenesis of the receptor-binding domain followed by in vitro selection, with wild-type ACE2 and the engineered decoy competing for binding sites, failed to find S mutants that discriminate in favor of the wild-type receptor. We conclude that resistance to engineered decoys will be rare and that decoys may be active against future outbreaks of SARS-associated betacoronaviruses. |*COVID-19 Drug Treatment[MESH] |*Protein Engineering[MESH] |Angiotensin-Converting Enzyme 2/*chemistry/genetics/metabolism/therapeutic use[MESH] |Animals[MESH] |Cell Line[MESH] |Chiroptera[MESH] |Humans[MESH] |Mutagenesis[MESH] |Protein Domains[MESH]An engineered decoy receptor for SARS-CoV-2 broadly binds protein S se(epmc).pdf DeepDyve Pubget Overpricing