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10.1097/JOM.0000000000002176

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suck abstract from ncbi


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pmid33596026      J+Occup+Environ+Med 2021 ; 63 (7): 541-547
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  • Saliva Pooling Strategy for the Large-Scale Detection of SARS-CoV-2, Through Working-Groups Testing of Asymptomatic Subjects for Potential Applications in Different Workplaces #MMPMID33596026
  • Giron-Perez DA; Ruiz-Manzano RA; Benitez-Trinidad AB; Ventura-Ramon GH; Covantes-Rosales CE; Ojeda-Duran AJ; Mercado-Salgado U; Toledo-Ibarra GA; Diaz-Resendiz KJ; Giron-Perez MI
  • J Occup Environ Med 2021[Jul]; 63 (7): 541-547 PMID33596026show ga
  • OBJECTIVE: To perform an improved large-scale SARS-CoV-2 detection on pooled tests of asymptomatic workers. METHODS: qRT-PCR validation of the SARS-CoV-2 detection in salivae samples and saliva pools and working-group saliva pooling and testing for SARS-CoV-2. RESULTS: We found a high Cycle threshold correlation (r = 0.9099) between swabs and saliva samples. Then, through the pooling strategy, we detected that 18/360 (5%) of individual saliva samples were SARS-CoV-2 positive. Saliva-pooling efficiency (360 of test sample/30 individual PCR) was higher (5.45) than the reported for swabbing group-testing and we spared 82% of the PCR reagents as well as sampling and personal protection equipment. CONCLUSION: Through this simplified and less expensive procedure, we detected in a short time asymptomatic-infected SARS-CoV-2-carriers that were isolated from their co-workers, thus, this methodology can be implemented in different workplaces to ensure consumers that employees are not infectious.
  • |Asymptomatic Infections[MESH]
  • |COVID-19 Nucleic Acid Testing/*methods[MESH]
  • |COVID-19 Testing/*methods[MESH]
  • |COVID-19/*diagnosis/prevention & control/virology[MESH]
  • |Contact Tracing[MESH]
  • |Humans[MESH]
  • |RNA, Viral/isolation & purification[MESH]
  • |SARS-CoV-2/genetics/*isolation & purification[MESH]
  • |Saliva/*virology[MESH]
  • |Sensitivity and Specificity[MESH]
  • |Specimen Handling[MESH]


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