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10.1002/biot.202100040 http://scihub22266oqcxt.onion/10.1002/biot.202100040 33595922!ä!33595922 Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=33595922&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biotechnol+J 2021 ; 16 (6): e2100040 Nephropedia Template TP {{tp|p=33595922|t=2021. Detection of the SARS-CoV-2 D614G mutation using engineered Cas12a guide RNA |pdf=|usr=}}{{33595922}} gab.com Text Detection of the SARS-CoV-2 D614G mutation using engineered Cas12a guide RNA JO: Biotechnol J http://www.kidney.de/pget.php?&tw=1&pmid=33595922 #FOAvip Detection of pathogens with single-nucleotide variations is indispensable for the disease tracing, but remains technically challenging. The D614G mutation in the SARS-CoV-2 spike protein is known to markedly enhance viral infectivity but is difficult to detect. Here, we report an effective approach called "synthetic mismatch integrated crRNA guided Cas12a detection" (symRNA-Cas12a) to detect the D614 and G614 variants effectively. Using this method, we systemically screened a pool of crRNAs that contain all the possible nucleotide substitutions covering the -2 to +2 positions around the mutation and identify one crRNA that can efficiently increase the detection specificity by 13-fold over the ancestral crRNA. With this selected crRNA, the symRNA-Cas12a assay can detect as low as 10 copies of synthetic mutant RNA and the results are confirmed to be accurate by Sanger sequencing. Overall, we have developed the symRNA-Cas12a method to specifically, sensitively and rapidly detect the SARS-CoV-2 D614G mutation. Twit Text FOAVip Detection of the SARS-CoV-2 D614G mutation using engineered Cas12a guide RNA ????Biotechnol J http://www.kidney.de/pget.php?&tw=1&pmid=33595922 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33595922 #FOAvip English Wikipedia <ref>{{Cite pmid|33595922}}</ref> Detection of the SARS-CoV-2 D614G mutation using engineered Cas12a guide RNA #MMPMID33595922 Meng Q; Wang X; Wang Y; Dang L; Liu X; Ma X; Chi T; Wang X; Zhao Q; Yang G; Liu M; Huang X; Ma P Biotechnol J 2021[Jun]; 16 (6): e2100040 PMID33595922show ga Detection of pathogens with single-nucleotide variations is indispensable for the disease tracing, but remains technically challenging. The D614G mutation in the SARS-CoV-2 spike protein is known to markedly enhance viral infectivity but is difficult to detect. Here, we report an effective approach called "synthetic mismatch integrated crRNA guided Cas12a detection" (symRNA-Cas12a) to detect the D614 and G614 variants effectively. Using this method, we systemically screened a pool of crRNAs that contain all the possible nucleotide substitutions covering the -2 to +2 positions around the mutation and identify one crRNA that can efficiently increase the detection specificity by 13-fold over the ancestral crRNA. With this selected crRNA, the symRNA-Cas12a assay can detect as low as 10 copies of synthetic mutant RNA and the results are confirmed to be accurate by Sanger sequencing. Overall, we have developed the symRNA-Cas12a method to specifically, sensitively and rapidly detect the SARS-CoV-2 D614G mutation. |*COVID-19[MESH] |*RNA, Guide, CRISPR-Cas Systems[MESH] |CRISPR-Cas Systems[MESH] |Humans[MESH] |Mutation[MESH] |SARS-CoV-2[MESH]Detection of the SARS-CoV-2 D614G mutation using engineered Cas12a gui(epmc).pdf DeepDyve Pubget Overpricing