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10.11613/BM.2021.010708 http://scihub22266oqcxt.onion/10.11613/BM.2021.010708 33594297!7852303!33594297     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biochem+Med+(Zagreb) 2021 ; 31 (1): 010708 Nephropedia Template TP {{tp|p=33594297|t=2021. Comparison of diagnostic accuracy for eight SARS-CoV-2 serological assays |pdf=|usr=}}{{33594297}} gab.com Text Comparison of diagnostic accuracy for eight SARS-CoV-2 serological assays JO: Biochem Med (Zagreb) http://www.kidney.de/pget.php?&tw=1&pmid=33594297 #FOAvip INTRODUCTION: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) serological tests have been suggested as an additional diagnostic tool in highly suspected cases with a negative molecular test and determination of seroprevalence in population. We compared the diagnostic performance of eight commercial serological assays for IgA, IgM, and IgG antibodies to the SARS-CoV-2 virus. MATERIALS AND METHODS: The comparison study was performed on a total of 76 serum samples: 30 SARS-CoV-2 polymerase chain reaction (PCR)-negative and 46 SARS-CoV-2 PCR-positive patients with asymptomatic to severe disease and symptoms duration from 3-30 days. The study included: three rapid lateral flow immunochromatographic assays (LFIC), two enzyme-linked immunosorbent assays (ELISA), and three chemiluminescence immunoassays (CLIA). RESULTS: Agreement between IgM assays were minimal to moderate (kappa 0.26 to 0.63) and for IgG moderate to excellent (kappa 0.72 to 0.92). Sensitivities improved with > 10 days of symptoms and were: 30% to 89% for IgM; 89% to 100% for IgG; 96% for IgA; 100% for IgA/IgM combination; 96% for total antibodies. Overall specificities were: 90% to 100% for IgM; 85% to 100% for IgG; 90% for IgA; 70% for IgA/IgM combination; 100% for total antibodies. Diagnostic accuracy for IgG ELISA and CIA assays were excellent (AUC >/= 0.90), without significant difference. IgA showed significantly better diagnostic accuracy than IgM (P < 0.001). CONCLUSION: There is high variability between IgM assays independently of the assay format, while IgG assays showed moderate to perfect agreement. The appropriate time for testing is crucial for the proper immunity investigation. Twit Text FOAVip Comparison of diagnostic accuracy for eight SARS-CoV-2 serological assays ????Biochem Med (Zagreb) http://www.kidney.de/pget.php?&tw=1&pmid=33594297 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33594297 #FOAvip English Wikipedia <ref>{{Cite pmid|33594297}}</ref> Comparison of diagnostic accuracy for eight SARS-CoV-2 serological assays #MMPMID33594297 Tesija Kuna A; Hanzek M; Vukasovic I; Nikolac Gabaj N; Vidranski V; Celap I; Miler M; Stancin N; Simac B; Zivkovic M; Zarak M; Kmet M; Jovanovic M; Tadinac S; Supraha Goreta S; Perisa J; Samija I; Stefanovic M Biochem Med (Zagreb) 2021[Feb]; 31 (1): 010708 PMID33594297show ga INTRODUCTION: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) serological tests have been suggested as an additional diagnostic tool in highly suspected cases with a negative molecular test and determination of seroprevalence in population. We compared the diagnostic performance of eight commercial serological assays for IgA, IgM, and IgG antibodies to the SARS-CoV-2 virus. MATERIALS AND METHODS: The comparison study was performed on a total of 76 serum samples: 30 SARS-CoV-2 polymerase chain reaction (PCR)-negative and 46 SARS-CoV-2 PCR-positive patients with asymptomatic to severe disease and symptoms duration from 3-30 days. The study included: three rapid lateral flow immunochromatographic assays (LFIC), two enzyme-linked immunosorbent assays (ELISA), and three chemiluminescence immunoassays (CLIA). RESULTS: Agreement between IgM assays were minimal to moderate (kappa 0.26 to 0.63) and for IgG moderate to excellent (kappa 0.72 to 0.92). Sensitivities improved with > 10 days of symptoms and were: 30% to 89% for IgM; 89% to 100% for IgG; 96% for IgA; 100% for IgA/IgM combination; 96% for total antibodies. Overall specificities were: 90% to 100% for IgM; 85% to 100% for IgG; 90% for IgA; 70% for IgA/IgM combination; 100% for total antibodies. Diagnostic accuracy for IgG ELISA and CIA assays were excellent (AUC >/= 0.90), without significant difference. IgA showed significantly better diagnostic accuracy than IgM (P < 0.001). CONCLUSION: There is high variability between IgM assays independently of the assay format, while IgG assays showed moderate to perfect agreement. The appropriate time for testing is crucial for the proper immunity investigation. |COVID-19 Testing/*methods/*standards[MESH] |COVID-19/*diagnosis/virology[MESH] |Humans[MESH] |SARS-CoV-2/isolation & purification[MESH] |Sensitivity and Specificity[MESH]Comparison of diagnostic accuracy for eight SARS-CoV-2 serological ass(epmc).pdf DeepDyve Pubget Overpricing