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10.1371/journal.pone.0246302 http://scihub22266oqcxt.onion/10.1371/journal.pone.0246302 33591986!7886139!33591986     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       PLoS+One 2021 ; 16 (2): e0246302 Nephropedia Template TP {{tp|p=33591986|t=2021. Evaluation of two automated low-cost RNA extraction protocols for SARS-CoV-2 detection |pdf=|usr=}}{{33591986}} gab.com Text Evaluation of two automated low-cost RNA extraction protocols for SARS-CoV-2 detection JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=33591986 #FOAvip BACKGROUND: Two automatable in-house protocols for high-troughput RNA extraction from nasopharyngeal swabs for SARS-CoV-2 detection have been evaluated. METHODS: One hundred forty one SARS-CoV-2 positive samples were collected during a period of 10-days. In-house protocols were based on extraction with magnetic beads and designed to be used with either the Opentrons OT-2 (OT-2in-house) liquid handling robot or the MagMAXTM Express-96 system (MMin-house). Both protocols were tested in parallel with a commercial kit that uses the MagMAXTM system (MMkit). Nucleic acid extraction efficiencies were calculated from a SARS-CoV-2 DNA positive control. RESULTS: No significant differences were found between both in-house protocols and the commercial kit in their performance to detect positive samples. The MMkit was the most efficient although the MMin-house presented, in average, lower Cts than the other two. In-house protocols allowed to save between 350euro and 400euro for every 96 extracted samples compared to the commercial kit. CONCLUSION: The protocols described harness the use of easily available reagents and an open-source liquid handling system and are suitable for SARS-CoV-2 detection in high throughput facilities. Twit Text FOAVip Evaluation of two automated low-cost RNA extraction protocols for SARS-CoV-2 detection ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=33591986 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33591986 #FOAvip English Wikipedia <ref>{{Cite pmid|33591986}}</ref> Evaluation of two automated low-cost RNA extraction protocols for SARS-CoV-2 detection #MMPMID33591986 Lazaro-Perona F; Rodriguez-Antolin C; Alguacil-Guillen M; Gutierrez-Arroyo A; Mingorance J; Garcia-Rodriguez J PLoS One 2021[]; 16 (2): e0246302 PMID33591986show ga BACKGROUND: Two automatable in-house protocols for high-troughput RNA extraction from nasopharyngeal swabs for SARS-CoV-2 detection have been evaluated. METHODS: One hundred forty one SARS-CoV-2 positive samples were collected during a period of 10-days. In-house protocols were based on extraction with magnetic beads and designed to be used with either the Opentrons OT-2 (OT-2in-house) liquid handling robot or the MagMAXTM Express-96 system (MMin-house). Both protocols were tested in parallel with a commercial kit that uses the MagMAXTM system (MMkit). Nucleic acid extraction efficiencies were calculated from a SARS-CoV-2 DNA positive control. RESULTS: No significant differences were found between both in-house protocols and the commercial kit in their performance to detect positive samples. The MMkit was the most efficient although the MMin-house presented, in average, lower Cts than the other two. In-house protocols allowed to save between 350euro and 400euro for every 96 extracted samples compared to the commercial kit. CONCLUSION: The protocols described harness the use of easily available reagents and an open-source liquid handling system and are suitable for SARS-CoV-2 detection in high throughput facilities. |Automation, Laboratory/economics/*methods/standards[MESH] |COVID-19 Nucleic Acid Testing/economics/*methods/standards[MESH] |Costs and Cost Analysis[MESH] |Humans[MESH] |RNA, Viral/chemistry/genetics/*standards[MESH] |Reagent Kits, Diagnostic/economics/standards[MESH]Evaluation of two automated low-cost RNA extraction protocols for SARS(epmc).pdf DeepDyve Pubget Overpricing