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10.1002/ptr.7030

http://scihub22266oqcxt.onion/10.1002/ptr.7030
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33587321!8013958!33587321
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suck abstract from ncbi


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pmid33587321      Phytother+Res 2021 ; 35 (6): 3194-3204
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  • Oroxylin A is a severe acute respiratory syndrome coronavirus 2-spiked pseudotyped virus blocker obtained from Radix Scutellariae using angiotensin-converting enzyme II/cell membrane chromatography #MMPMID33587321
  • Gao J; Ding Y; Wang Y; Liang P; Zhang L; Liu R
  • Phytother Res 2021[Jun]; 35 (6): 3194-3204 PMID33587321show ga
  • The current worldwide outbreak of the coronavirus disease 2019 (COVID-19) has been declared a public health emergency. The angiotensin-converting enzyme II (ACE2) has been reported as the primary host-cell receptor for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative virus of COVID-19. In this study, we screened ACE2 ligands from Radix Scutellariae and investigated its suppressive effect on SARS-CoV-2 spiked pseudotyped virus in vitro. HEK293T cells stably expressing ACE2 receptors (ACE2 cells) were used to provide the receptor for the ACE2/cell membrane chromatography (CMC) method used for analysis. The SARS-CoV-2-spiked pseudotyped virus was used to examine the anti-viropexis effect of the screened compounds in ACE2 cells. Molecular docking and the surface plasmon resonance (SPR) assay were used to determine the binding properties. Oroxylin A exhibited an appreciable suppressive effect against the entrance of the SARS-CoV-2-spiked pseudotyped virus into ACE2 cells, which showed good binding to ACE2 as determined using SPR and CMC. Oroxylin A was shown to be a potential candidate in the treatment for COVID-19 by virtue of its blocking the entrance of SARS-CoV-2 into ACE2 cells by specifically binding to the ACE2 receptor.
  • |*COVID-19 Drug Treatment[MESH]
  • |Angiotensin-Converting Enzyme 2/metabolism[MESH]
  • |Cell Membrane/metabolism[MESH]
  • |Chromatography[MESH]
  • |Flavonoids/*pharmacology[MESH]
  • |HEK293 Cells[MESH]
  • |Humans[MESH]
  • |Molecular Docking Simulation[MESH]
  • |Protein Binding/drug effects[MESH]
  • |SARS-CoV-2/*drug effects[MESH]


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