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10.1016/j.virol.2021.01.004 http://scihub22266oqcxt.onion/10.1016/j.virol.2021.01.004 33582454!7871913!33582454     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Virology 2021 ; 557 (ä): 15-22 Nephropedia Template TP {{tp|p=33582454|t=2021. Expression, purification and immunological characterization of recombinant nucleocapsid protein fragment from SARS-CoV-2 |pdf=|usr=}}{{33582454}} gab.com Text Expression, purification and immunological characterization of recombinant nucleocapsid protein fragment from SARS-CoV-2 JO: Virology http://www.kidney.de/pget.php?&tw=1&pmid=33582454 #FOAvip Serological testing is important method for diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Nucleocapsid (N) protein is the most abundant virus derived protein and strong immunogen. We aimed to find its efficient, low-cost production. SARS-CoV-2 recombinant fragment of nucleocapsid protein (rfNP; 58-419 aa) was expressed in E. coli in soluble form, purified and characterized biochemically and immunologically. Purified rfNP has secondary structure of full-length recombinant N protein, with high percentage of disordered structure (34.2%) and of beta-sheet (40.7%). rfNP was tested in immunoblot using sera of COVID-19 convalescent patients. ELISA was optimized with sera of RT-PCR confirmed positive symptomatic patients and healthy individuals. IgG detection sensitivity was 96% (47/50) and specificity 97% (67/68), while IgM detection was slightly lower (94% and 96.5%, respectively). Cost-effective approach for soluble recombinant N protein fragment production was developed, with reliable IgG and IgM antibodies detection of SARS-CoV-2 infection. Twit Text FOAVip Expression, purification and immunological characterization of recombinant nucleocapsid protein fragment from SARS-CoV-2 ????Virology http://www.kidney.de/pget.php?&tw=1&pmid=33582454 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33582454 #FOAvip English Wikipedia <ref>{{Cite pmid|33582454}}</ref> Expression, purification and immunological characterization of recombinant nucleocapsid protein fragment from SARS-CoV-2 #MMPMID33582454 Djukic T; Mladenovic M; Stanic-Vucinic D; Radosavljevic J; Smiljanic K; Sabljic L; Devic M; Cujic D; Vasovic T; Simovic A; Radomirovic M; Cirkovic Velickovic T Virology 2021[May]; 557 (ä): 15-22 PMID33582454show ga Serological testing is important method for diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Nucleocapsid (N) protein is the most abundant virus derived protein and strong immunogen. We aimed to find its efficient, low-cost production. SARS-CoV-2 recombinant fragment of nucleocapsid protein (rfNP; 58-419 aa) was expressed in E. coli in soluble form, purified and characterized biochemically and immunologically. Purified rfNP has secondary structure of full-length recombinant N protein, with high percentage of disordered structure (34.2%) and of beta-sheet (40.7%). rfNP was tested in immunoblot using sera of COVID-19 convalescent patients. ELISA was optimized with sera of RT-PCR confirmed positive symptomatic patients and healthy individuals. IgG detection sensitivity was 96% (47/50) and specificity 97% (67/68), while IgM detection was slightly lower (94% and 96.5%, respectively). Cost-effective approach for soluble recombinant N protein fragment production was developed, with reliable IgG and IgM antibodies detection of SARS-CoV-2 infection. |Amino Acid Sequence[MESH] |Antibodies, Viral/*blood[MESH] |COVID-19 Serological Testing/methods[MESH] |COVID-19/blood/*diagnosis/immunology[MESH] |Case-Control Studies[MESH] |Cloning, Molecular[MESH] |Coronavirus Nucleocapsid Proteins/genetics/*immunology[MESH] |Enzyme-Linked Immunosorbent Assay/*methods/standards[MESH] |Escherichia coli/genetics/metabolism[MESH] |Gene Expression[MESH] |Genetic Vectors/chemistry/metabolism[MESH] |Immune Sera/chemistry[MESH] |Immunoglobulin G/*blood[MESH] |Immunoglobulin M/blood[MESH] |Phosphoproteins/genetics/immunology[MESH] |Recombinant Proteins/genetics/immunology[MESH] |Reverse Transcriptase Polymerase Chain Reaction[MESH] |SARS-CoV-2/genetics/*immunology[MESH]Expression, purification and immunological characterization of recombi(epmc).pdf DeepDyve Pubget Overpricing