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10.1002/jmv.26874 http://scihub22266oqcxt.onion/10.1002/jmv.26874 33580518!8013543!33580518     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Med+Virol 2021 ; 93 (5): 3143-3151 Nephropedia Template TP {{tp|p=33580518|t=2021. Three salvianolic acids inhibit 2019-nCoV spike pseudovirus viropexis by binding to both its RBD and receptor ACE2 |pdf=|usr=}}{{33580518}} gab.com Text Three salvianolic acids inhibit 2019-nCoV spike pseudovirus viropexis by binding to both its RBD and receptor ACE2 JO: J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=33580518 #FOAvip Since December 2019, the new coronavirus (also known as severe acute respiratory syndrome coronavirus 2 [SARS-CoV-2, 2019-nCoV])-induced disease, COVID-19, has spread rapidly worldwide. Studies have reported that the traditional Chinese medicine Salvia miltiorrhiza possesses remarkable antiviral properties; however, the anti-coronaviral activity of its main components, salvianolic acid A (SAA), salvianolic acid B (SAB), and salvianolic acid C (SAC) is still debated. In this study, we used Cell Counting Kit-8 staining and flow cytometry to evaluate the toxicity of SAA, SAB, and SAC on ACE2 (angiotensin-converting enzyme 2) high-expressing HEK293T cells (ACE2(h) cells). We found that SAA, SAB, and SAC had a minor effect on the viability of ACE2(h) cells at concentrations below 100 muM. We further evaluated the binding capacity of SAA, SAB, and SAC to ACE2 and the spike protein of 2019-nCoV using molecular docking and surface plasmon resonance. They could bind to the receptor-binding domain (RBD) of the 2019-nCoV with a binding constant (K(D) ) of (3.82 +/- 0.43) e-6 M, (5.15 +/- 0.64)e-7 M, and (2.19 +/- 0.14)e-6 M; and bind to ACE2 with K(D) (4.08 +/- 0.61)e-7 M, (2.95 +/- 0.78)e-7 M, and (7.32 +/- 0.42)e-7 M, respectively. As a result, SAA, SAB, and SAC were determined to inhibit the entry of 2019-nCoV Spike pseudovirus with an EC(50) of 11.31, 6.22, and 10.14 muM on ACE2(h) cells, respectively. In conclusion, our study revealed that three Salvianolic acids can inhibit the entry of 2019-nCoV spike pseudovirus into ACE2(h) cells by binding to the RBD of the 2019-nCoV spike protein and ACE2 protein. Twit Text FOAVip Three salvianolic acids inhibit 2019-nCoV spike pseudovirus viropexis by binding to both its RBD and receptor ACE2 ????J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=33580518 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33580518 #FOAvip English Wikipedia <ref>{{Cite pmid|33580518}}</ref> Three salvianolic acids inhibit 2019-nCoV spike pseudovirus viropexis by binding to both its RBD and receptor ACE2 #MMPMID33580518 Hu S; Wang J; Zhang Y; Bai H; Wang C; Wang N; He L J Med Virol 2021[May]; 93 (5): 3143-3151 PMID33580518show ga Since December 2019, the new coronavirus (also known as severe acute respiratory syndrome coronavirus 2 [SARS-CoV-2, 2019-nCoV])-induced disease, COVID-19, has spread rapidly worldwide. Studies have reported that the traditional Chinese medicine Salvia miltiorrhiza possesses remarkable antiviral properties; however, the anti-coronaviral activity of its main components, salvianolic acid A (SAA), salvianolic acid B (SAB), and salvianolic acid C (SAC) is still debated. In this study, we used Cell Counting Kit-8 staining and flow cytometry to evaluate the toxicity of SAA, SAB, and SAC on ACE2 (angiotensin-converting enzyme 2) high-expressing HEK293T cells (ACE2(h) cells). We found that SAA, SAB, and SAC had a minor effect on the viability of ACE2(h) cells at concentrations below 100 muM. We further evaluated the binding capacity of SAA, SAB, and SAC to ACE2 and the spike protein of 2019-nCoV using molecular docking and surface plasmon resonance. They could bind to the receptor-binding domain (RBD) of the 2019-nCoV with a binding constant (K(D) ) of (3.82 +/- 0.43) e-6 M, (5.15 +/- 0.64)e-7 M, and (2.19 +/- 0.14)e-6 M; and bind to ACE2 with K(D) (4.08 +/- 0.61)e-7 M, (2.95 +/- 0.78)e-7 M, and (7.32 +/- 0.42)e-7 M, respectively. As a result, SAA, SAB, and SAC were determined to inhibit the entry of 2019-nCoV Spike pseudovirus with an EC(50) of 11.31, 6.22, and 10.14 muM on ACE2(h) cells, respectively. In conclusion, our study revealed that three Salvianolic acids can inhibit the entry of 2019-nCoV spike pseudovirus into ACE2(h) cells by binding to the RBD of the 2019-nCoV spike protein and ACE2 protein. |Alkenes/chemistry/*pharmacology[MESH] |Angiotensin-Converting Enzyme 2/chemistry/*metabolism[MESH] |Benzofurans/chemistry/*pharmacology[MESH] |COVID-19 Drug Treatment[MESH] |Caffeic Acids/chemistry/*pharmacology[MESH] |Cell Survival[MESH] |HEK293 Cells[MESH] |Humans[MESH] |Lactates/chemistry/*pharmacology[MESH] |Molecular Structure[MESH] |Polyphenols/chemistry/*pharmacology[MESH] |Protein Binding[MESH] |SARS-CoV-2/*drug effects[MESH] |Spike Glycoprotein, Coronavirus/chemistry/*metabolism[MESH]Three salvianolic acids inhibit 2019-nCoV spike pseudovirus viropexis (epmc).pdf DeepDyve Pubget Overpricing