Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.3390/ijms22041772 http://scihub22266oqcxt.onion/10.3390/ijms22041772 33578973!7916667!33578973     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Int+J+Mol+Sci 2021 ; 22 (4): ä Nephropedia Template TP {{tp|p=33578973|t=2021. Detection of Coronaviruses Using RNA Toehold Switch Sensors |pdf=|usr=}}{{33578973}} gab.com Text Detection of Coronaviruses Using RNA Toehold Switch Sensors JO: Int J Mol Sci http://www.kidney.de/pget.php?&tw=1&pmid=33578973 #FOAvip A rapid, sensitive and simple point-of-care (POC) nucleic acid diagnostic test is needed to prevent spread of infectious diseases. Paper-based toehold reaction, a recently emerged colorimetric POC nucleic acid diagnostic test, has been widely used for pathogen detection and microbiome profiling. Here, we introduce an amplification method called reverse transcription loop-mediated amplification (RT-LAMP) prior to the toehold reaction and modify it to enable more sensitive and faster colorimetric detection of RNA viruses. We show that incorporating the modified RT-LAMP to the toehold reaction detects as few as 120 copies of coronavirus RNA in 70 min. Cross-reactivity test against other coronaviruses indicates this toehold reaction with the modified RT-LAMP is highly specific to the target RNA. Overall, the paper-based toehold switch sensors with the modified RT-LAMP allow fast, sensitive, specific and colorimetric coronavirus detection. Twit Text FOAVip Detection of Coronaviruses Using RNA Toehold Switch Sensors ????Int J Mol Sci http://www.kidney.de/pget.php?&tw=1&pmid=33578973 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33578973 #FOAvip English Wikipedia <ref>{{Cite pmid|33578973}}</ref> Detection of Coronaviruses Using RNA Toehold Switch Sensors #MMPMID33578973 Park S; Lee JW Int J Mol Sci 2021[Feb]; 22 (4): ä PMID33578973show ga A rapid, sensitive and simple point-of-care (POC) nucleic acid diagnostic test is needed to prevent spread of infectious diseases. Paper-based toehold reaction, a recently emerged colorimetric POC nucleic acid diagnostic test, has been widely used for pathogen detection and microbiome profiling. Here, we introduce an amplification method called reverse transcription loop-mediated amplification (RT-LAMP) prior to the toehold reaction and modify it to enable more sensitive and faster colorimetric detection of RNA viruses. We show that incorporating the modified RT-LAMP to the toehold reaction detects as few as 120 copies of coronavirus RNA in 70 min. Cross-reactivity test against other coronaviruses indicates this toehold reaction with the modified RT-LAMP is highly specific to the target RNA. Overall, the paper-based toehold switch sensors with the modified RT-LAMP allow fast, sensitive, specific and colorimetric coronavirus detection. |Coronavirus Infections/*diagnosis[MESH] |Coronavirus/genetics/*isolation & purification[MESH] |Diagnostic Tests, Routine[MESH] |Humans[MESH] |Molecular Diagnostic Techniques[MESH] |Nucleic Acid Amplification Techniques[MESH] |Point-of-Care Testing[MESH] |RNA, Viral/genetics[MESH]Detection of Coronaviruses Using RNA Toehold Switch Sensors (epmc).pdf DeepDyve Pubget Overpricing