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10.1021/acs.analchem.0c04952 http://scihub22266oqcxt.onion/10.1021/acs.analchem.0c04952 33577736!ä!33577736 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 298.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Anal+Chem 2021 ; 93 (8): 3976-3986 Nephropedia Template TP {{tp|p=33577736|t=2021. Diffusion and Relaxation Edited Proton NMR Spectroscopy of Plasma Reveals a High-Fidelity Supramolecular Biomarker Signature of SARS-CoV-2 Infection |pdf=|usr=}}{{33577736}} gab.com Text Diffusion and Relaxation Edited Proton NMR Spectroscopy of Plasma Reveals a High-Fidelity Supramolecular Biomarker Signature of SARS-CoV-2 Infection JO: Anal Chem http://www.kidney.de/pget.php?&tw=1&pmid=33577736 #FOAvip We have applied nuclear magnetic resonance spectroscopy based plasma phenotyping to reveal diagnostic molecular signatures of SARS-CoV-2 infection via combined diffusional and relaxation editing (DIRE). We compared plasma from healthy age-matched controls (n = 26) with SARS-CoV-2 negative non-hospitalized respiratory patients and hospitalized respiratory patients (n = 23 and 11 respectively) with SARS-CoV-2 rRT-PCR positive respiratory patients (n = 17, with longitudinal sampling time-points). DIRE data were modelled using principal component analysis and orthogonal projections to latent structures discriminant analysis (O-PLS-DA), with statistical cross-validation indices indicating excellent model generalization for the classification of SARS-CoV-2 positivity for all comparator groups (area under the receiver operator characteristic curve = 1). DIRE spectra show biomarker signal combinations conferred by differential concentrations of metabolites with selected molecular mobility properties. These comprise the following: (a) composite N-acetyl signals from alpha-1-acid glycoprotein and other glycoproteins (designated GlycA and GlycB) that were elevated in SARS-CoV-2 positive patients [p = 2.52 x 10(-10) (GlycA) and 1.25 x 10(-9) (GlycB) vs controls], (b) two diagnostic supramolecular phospholipid composite signals that were identified (SPC-A and SPC-B) from the -(+)N-(CH(3))(3) choline headgroups of lysophosphatidylcholines carried on plasma glycoproteins and from phospholipids in high-density lipoprotein subfractions (SPC-A) together with a phospholipid component of low-density lipoprotein (SPC-B). The integrals of the summed SPC signals (SPC(total)) were reduced in SARS-CoV-2 positive patients relative to both controls (p = 1.40 x 10(-7)) and SARS-CoV-2 negative patients (p = 4.52 x 10(-8)) but were not significantly different between controls and SARS-CoV-2 negative patients. The identity of the SPC signal components was determined using one and two dimensional diffusional, relaxation, and statistical spectroscopic experiments. The SPC(total)/GlycA ratios were also significantly different for control versus SARS-CoV-2 positive patients (p = 1.23 x 10(-10)) and for SARS-CoV-2 negatives versus positives (p = 1.60 x 10(-9)). Thus, plasma SPC(total) and SPC(total)/GlycA are proposed as sensitive molecular markers for SARS-CoV-2 positivity that could effectively augment current COVID-19 diagnostics and may have value in functional assessment of the disease recovery process in patients with long-term symptoms. Twit Text FOAVip Diffusion and Relaxation Edited Proton NMR Spectroscopy of Plasma Reveals a High-Fidelity Supramolecular Biomarker Signature of SARS-CoV-2 Infection ????Anal Chem http://www.kidney.de/pget.php?&tw=1&pmid=33577736 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33577736 #FOAvip English Wikipedia <ref>{{Cite pmid|33577736}}</ref> Diffusion and Relaxation Edited Proton NMR Spectroscopy of Plasma Reveals a High-Fidelity Supramolecular Biomarker Signature of SARS-CoV-2 Infection #MMPMID33577736 Lodge S; Nitschke P; Kimhofer T; Wist J; Bong SH; Loo RL; Masuda R; Begum S; Richards T; Lindon JC; Bermel W; Reinsperger T; Schaefer H; Spraul M; Holmes E; Nicholson JK Anal Chem 2021[Mar]; 93 (8): 3976-3986 PMID33577736show ga We have applied nuclear magnetic resonance spectroscopy based plasma phenotyping to reveal diagnostic molecular signatures of SARS-CoV-2 infection via combined diffusional and relaxation editing (DIRE). We compared plasma from healthy age-matched controls (n = 26) with SARS-CoV-2 negative non-hospitalized respiratory patients and hospitalized respiratory patients (n = 23 and 11 respectively) with SARS-CoV-2 rRT-PCR positive respiratory patients (n = 17, with longitudinal sampling time-points). DIRE data were modelled using principal component analysis and orthogonal projections to latent structures discriminant analysis (O-PLS-DA), with statistical cross-validation indices indicating excellent model generalization for the classification of SARS-CoV-2 positivity for all comparator groups (area under the receiver operator characteristic curve = 1). DIRE spectra show biomarker signal combinations conferred by differential concentrations of metabolites with selected molecular mobility properties. These comprise the following: (a) composite N-acetyl signals from alpha-1-acid glycoprotein and other glycoproteins (designated GlycA and GlycB) that were elevated in SARS-CoV-2 positive patients [p = 2.52 x 10(-10) (GlycA) and 1.25 x 10(-9) (GlycB) vs controls], (b) two diagnostic supramolecular phospholipid composite signals that were identified (SPC-A and SPC-B) from the -(+)N-(CH(3))(3) choline headgroups of lysophosphatidylcholines carried on plasma glycoproteins and from phospholipids in high-density lipoprotein subfractions (SPC-A) together with a phospholipid component of low-density lipoprotein (SPC-B). The integrals of the summed SPC signals (SPC(total)) were reduced in SARS-CoV-2 positive patients relative to both controls (p = 1.40 x 10(-7)) and SARS-CoV-2 negative patients (p = 4.52 x 10(-8)) but were not significantly different between controls and SARS-CoV-2 negative patients. The identity of the SPC signal components was determined using one and two dimensional diffusional, relaxation, and statistical spectroscopic experiments. The SPC(total)/GlycA ratios were also significantly different for control versus SARS-CoV-2 positive patients (p = 1.23 x 10(-10)) and for SARS-CoV-2 negatives versus positives (p = 1.60 x 10(-9)). Thus, plasma SPC(total) and SPC(total)/GlycA are proposed as sensitive molecular markers for SARS-CoV-2 positivity that could effectively augment current COVID-19 diagnostics and may have value in functional assessment of the disease recovery process in patients with long-term symptoms. |Aged[MESH] |Biomarkers/blood[MESH] |COVID-19/blood/*diagnosis[MESH] |Female[MESH] |Humans[MESH] |Male[MESH] |Middle Aged[MESH] |Multivariate Analysis[MESH] |Nuclear Magnetic Resonance, Biomolecular/methods[MESH] |Orosomucoid/*analysis/chemistry[MESH] |Phospholipids/*blood/chemistry[MESH] |Proton Magnetic Resonance Spectroscopy/methods/statistics & numerical data[MESH] |ROC Curve[MESH]Diffusion and Relaxation Edited Proton NMR Spectroscopy of Plasma Reve(epmc).pdf DeepDyve Pubget Overpricing