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10.1038/s41467-021-21213-4 http://scihub22266oqcxt.onion/10.1038/s41467-021-21213-4 33574281!7878750!33574281     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2021 ; 12 (1): 961 Nephropedia Template TP {{tp|p=33574281|t=2021. A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry |pdf=|usr=}}{{33574281}} gab.com Text A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=33574281 #FOAvip The global spread of SARS-CoV-2 is posing major public health challenges. One feature of SARS-CoV-2 spike protein is the insertion of multi-basic residues at the S1/S2 subunit cleavage site. Here, we find that the virus with intact spike (Sfull) preferentially enters cells via fusion at the plasma membrane, whereas a clone (Sdel) with deletion disrupting the multi-basic S1/S2 site utilizes an endosomal entry pathway. Using Sdel as model, we perform a genome-wide CRISPR screen and identify several endosomal entry-specific regulators. Experimental validation of hits from the CRISPR screen shows that host factors regulating the surface expression of angiotensin-converting enzyme 2 (ACE2) affect entry of Sfull virus. Animal-to-animal transmission with the Sdel virus is reduced compared to Sfull in the hamster model. These findings highlight the critical role of the S1/S2 boundary of SARS-CoV-2 spike protein in modulating virus entry and transmission and provide insights into entry of coronaviruses. Twit Text FOAVip A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=33574281 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33574281 #FOAvip English Wikipedia <ref>{{Cite pmid|33574281}}</ref> A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry #MMPMID33574281 Zhu Y; Feng F; Hu G; Wang Y; Yu Y; Zhu Y; Xu W; Cai X; Sun Z; Han W; Ye R; Qu D; Ding Q; Huang X; Chen H; Xu W; Xie Y; Cai Q; Yuan Z; Zhang R Nat Commun 2021[Feb]; 12 (1): 961 PMID33574281show ga The global spread of SARS-CoV-2 is posing major public health challenges. One feature of SARS-CoV-2 spike protein is the insertion of multi-basic residues at the S1/S2 subunit cleavage site. Here, we find that the virus with intact spike (Sfull) preferentially enters cells via fusion at the plasma membrane, whereas a clone (Sdel) with deletion disrupting the multi-basic S1/S2 site utilizes an endosomal entry pathway. Using Sdel as model, we perform a genome-wide CRISPR screen and identify several endosomal entry-specific regulators. Experimental validation of hits from the CRISPR screen shows that host factors regulating the surface expression of angiotensin-converting enzyme 2 (ACE2) affect entry of Sfull virus. Animal-to-animal transmission with the Sdel virus is reduced compared to Sfull in the hamster model. These findings highlight the critical role of the S1/S2 boundary of SARS-CoV-2 spike protein in modulating virus entry and transmission and provide insights into entry of coronaviruses. |*CRISPR-Cas Systems[MESH] |*Genome-Wide Association Study[MESH] |*Host-Pathogen Interactions[MESH] |*Virus Internalization[MESH] |A549 Cells[MESH] |Angiotensin-Converting Enzyme 2/genetics/metabolism[MESH] |Animals[MESH] |COVID-19/genetics/*virology[MESH] |Chlorocebus aethiops[MESH] |Disease Models, Animal[MESH] |Endosomes/virology[MESH] |HeLa Cells[MESH] |Humans[MESH] |Mesocricetus[MESH] |SARS-CoV-2/*physiology[MESH] |Serine Endopeptidases[MESH] |Spike Glycoprotein, Coronavirus/metabolism[MESH]A genome-wide CRISPR screen identifies host factors that regulate SARS(epmc).pdf DeepDyve Pubget Overpricing