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10.1016/j.diagmicrobio.2021.115334 http://scihub22266oqcxt.onion/10.1016/j.diagmicrobio.2021.115334 33571862!7987987!33571862     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=33571862&cmd=llinks): Failed to open stream: HTTP request failed! 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Understanding false positives and the detection of SARS-CoV-2 using the Cepheid Xpert Xpress SARS-CoV-2 and BD MAX SARS-CoV-2 assays |pdf=|usr=}}{{33571862}} gab.com Text Understanding false positives and the detection of SARS-CoV-2 using the Cepheid Xpert Xpress SARS-CoV-2 and BD MAX SARS-CoV-2 assays JO: Diagn Microbiol Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=33571862 #FOAvip Several real-time RT-PCR assays have received Emergency Use Authorization from the United States Food and Drug Administration. The BD MAX SARS-CoV-2 assay, run by the BD MAX system, is a qualitative test that detects the SARS-CoV-2 specific nucleocapsid phosphoprotein gene regions, N1 and N2. The human RNase P gene is used as the endogenous nucleic acid extraction control. The Cepheid Xpert(R) Xpress SARS-CoV-2 assay, run by the GeneXpert system, detects the pan-sarbecovirus E gene and the N2 region of the N gene. We evaluated the performance characteristics of the BD and Cepheid assays using matched patient samples. We also analyzed comparative Ct values for both assays using 183 positive samples tested at this facility. In addition, we mitigated reporting false positive results without relying on interpretive software. We found that both systems showed comparable sensitivity. We found an approximately 3.5% false positive rate from the BD MAX system results. Twit Text FOAVip Understanding false positives and the detection of SARS-CoV-2 using the Cepheid Xpert Xpress SARS-CoV-2 and BD MAX SARS-CoV-2 assays ????Diagn Microbiol Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=33571862 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33571862 #FOAvip English Wikipedia <ref>{{Cite pmid|33571862}}</ref> Understanding false positives and the detection of SARS-CoV-2 using the Cepheid Xpert Xpress SARS-CoV-2 and BD MAX SARS-CoV-2 assays #MMPMID33571862 Navarathna DH; Sharp S; Lukey J; Arenas M; Villas H; Wiley L; Englett I; Juan MRS; Jinadatha C Diagn Microbiol Infect Dis 2021[May]; 100 (1): 115334 PMID33571862show ga Several real-time RT-PCR assays have received Emergency Use Authorization from the United States Food and Drug Administration. The BD MAX SARS-CoV-2 assay, run by the BD MAX system, is a qualitative test that detects the SARS-CoV-2 specific nucleocapsid phosphoprotein gene regions, N1 and N2. The human RNase P gene is used as the endogenous nucleic acid extraction control. The Cepheid Xpert(R) Xpress SARS-CoV-2 assay, run by the GeneXpert system, detects the pan-sarbecovirus E gene and the N2 region of the N gene. We evaluated the performance characteristics of the BD and Cepheid assays using matched patient samples. We also analyzed comparative Ct values for both assays using 183 positive samples tested at this facility. In addition, we mitigated reporting false positive results without relying on interpretive software. We found that both systems showed comparable sensitivity. We found an approximately 3.5% false positive rate from the BD MAX system results. |COVID-19 Nucleic Acid Testing/*methods[MESH] |Coronavirus Envelope Proteins/genetics[MESH] |Coronavirus Nucleocapsid Proteins/genetics[MESH] |False Positive Reactions[MESH] |Humans[MESH] |Phosphoproteins/genetics[MESH]Understanding false positives and the detection of SARS-CoV-2 using th(epmc).pdf DeepDyve Pubget Overpricing