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10.1016/j.diagmicrobio.2021.115330

http://scihub22266oqcxt.onion/10.1016/j.diagmicrobio.2021.115330
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33571860!7846879!33571860
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suck abstract from ncbi


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pmid33571860      Diagn+Microbiol+Infect+Dis 2021 ; 100 (1): 115330
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  • Sample pooling is efficient in PCR testing of SARS-CoV-2: a study in 7400 healthcare professionals #MMPMID33571860
  • Estevez A; Catalan P; Alonso R; Marin M; Bouza E; Munoz P; Alcala L
  • Diagn Microbiol Infect Dis 2021[May]; 100 (1): 115330 PMID33571860show ga
  • SARS-CoV-2 pandemic shows the importance of having efficient virus diagnosis, especially in groups of particular relevance such as health care professionals, without involving a large economic expense. This is a prevalence study carried out in 7400 health care professionals in a 1350-bed hospital in Madrid, Spain. Pools of 10 samples were performed, using the Xpert(R) Xpress SARS-CoV-2 test for the diagnosis from clinical samples of nasopharyngeal exudate. A previous study was performed to evaluate the effect of the dilution in terms of sensitivity. The estimated sensitivity was over 95%. A total of 740 pools were performed, with a final result of 218 health care professionals being positive. Using the pooling system, the reagent cost reduction to the institution was 75.3%. It can be concluded that the described sample pooling system is a useful and efficient tool in the diagnosis of SARS-CoV-2 in certain groups, assuming a cost reduction without reducing the sensitivity.
  • |COVID-19 Nucleic Acid Testing/*methods[MESH]
  • |COVID-19/epidemiology[MESH]
  • |False Positive Reactions[MESH]
  • |Humans[MESH]
  • |Nasopharynx/virology[MESH]
  • |Personnel, Hospital/*statistics & numerical data[MESH]
  • |Polymerase Chain Reaction/*methods[MESH]
  • |Prevalence[MESH]
  • |Reproducibility of Results[MESH]
  • |Sensitivity and Specificity[MESH]
  • |Spain/epidemiology[MESH]


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