Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1371/journal.ppat.1009165 http://scihub22266oqcxt.onion/10.1371/journal.ppat.1009165 33571304!7877634!33571304     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       PLoS+Pathog 2021 ; 17 (2): e1009165 Nephropedia Template TP {{tp|p=33571304|t=2021. Multi-clonal SARS-CoV-2 neutralization by antibodies isolated from severe COVID-19 convalescent donors |pdf=|usr=}}{{33571304}} gab.com Text Multi-clonal SARS-CoV-2 neutralization by antibodies isolated from severe COVID-19 convalescent donors JO: PLoS Pathog http://www.kidney.de/pget.php?&tw=1&pmid=33571304 #FOAvip The interactions between antibodies, SARS-CoV-2 and immune cells contribute to the pathogenesis of COVID-19 and protective immunity. To understand the differences between antibody responses in mild versus severe cases of COVID-19, we analyzed the B cell responses in patients 1.5 months post SARS-CoV-2 infection. Severe, and not mild, infection correlated with high titers of IgG against Spike receptor binding domain (RBD) that were capable of ACE2:RBD inhibition. B cell receptor (BCR) sequencing revealed that VH3-53 was enriched during severe infection. Of the 22 antibodies cloned from two severe donors, six exhibited potent neutralization against authentic SARS-CoV-2, and inhibited syncytia formation. Using peptide libraries, competition ELISA and mutagenesis of RBD, we mapped the epitopes of the neutralizing antibodies (nAbs) to three different sites on the Spike. Finally, we used combinations of nAbs targeting different immune-sites to efficiently block SARS-CoV-2 infection. Analysis of 49 healthy BCR repertoires revealed that the nAbs germline VHJH precursors comprise up to 2.7% of all VHJHs. We demonstrate that severe COVID-19 is associated with unique BCR signatures and multi-clonal neutralizing responses that are relatively frequent in the population. Moreover, our data support the use of combination antibody therapy to prevent and treat COVID-19. Twit Text FOAVip Multi-clonal SARS-CoV-2 neutralization by antibodies isolated from severe COVID-19 convalescent donors ????PLoS Pathog http://www.kidney.de/pget.php?&tw=1&pmid=33571304 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33571304 #FOAvip English Wikipedia <ref>{{Cite pmid|33571304}}</ref> Multi-clonal SARS-CoV-2 neutralization by antibodies isolated from severe COVID-19 convalescent donors #MMPMID33571304 Mor M; Werbner M; Alter J; Safra M; Chomsky E; Lee JC; Hada-Neeman S; Polonsky K; Nowell CJ; Clark AE; Roitburd-Berman A; Ben-Shalom N; Navon M; Rafael D; Sharim H; Kiner E; Griffis ER; Gershoni JM; Kobiler O; Leibel SL; Zimhony O; Carlin AF; Yaari G; Dessau M; Gal-Tanamy M; Hagin D; Croker BA; Freund NT PLoS Pathog 2021[Feb]; 17 (2): e1009165 PMID33571304show ga The interactions between antibodies, SARS-CoV-2 and immune cells contribute to the pathogenesis of COVID-19 and protective immunity. To understand the differences between antibody responses in mild versus severe cases of COVID-19, we analyzed the B cell responses in patients 1.5 months post SARS-CoV-2 infection. Severe, and not mild, infection correlated with high titers of IgG against Spike receptor binding domain (RBD) that were capable of ACE2:RBD inhibition. B cell receptor (BCR) sequencing revealed that VH3-53 was enriched during severe infection. Of the 22 antibodies cloned from two severe donors, six exhibited potent neutralization against authentic SARS-CoV-2, and inhibited syncytia formation. Using peptide libraries, competition ELISA and mutagenesis of RBD, we mapped the epitopes of the neutralizing antibodies (nAbs) to three different sites on the Spike. Finally, we used combinations of nAbs targeting different immune-sites to efficiently block SARS-CoV-2 infection. Analysis of 49 healthy BCR repertoires revealed that the nAbs germline VHJH precursors comprise up to 2.7% of all VHJHs. We demonstrate that severe COVID-19 is associated with unique BCR signatures and multi-clonal neutralizing responses that are relatively frequent in the population. Moreover, our data support the use of combination antibody therapy to prevent and treat COVID-19. |*Antibodies, Monoclonal/genetics/immunology[MESH] |*Antibodies, Neutralizing/genetics/immunology[MESH] |*Antibodies, Viral/genetics/immunology[MESH] |*COVID-19/genetics/immunology[MESH] |*Convalescence[MESH] |*SARS-CoV-2/immunology[MESH] |*Spike Glycoprotein, Coronavirus/genetics/immunology[MESH] |Adult[MESH] |Aged[MESH] |Animals[MESH] |Chlorocebus aethiops[MESH] |Cloning, Molecular[MESH] |Epitope Mapping[MESH] |Epitopes/genetics/immunology[MESH] |Female[MESH] |Humans[MESH] |Immunoglobulin G/genetics/immunology[MESH] |Male[MESH] |Middle Aged[MESH]Multi-clonal SARS-CoV-2 neutralization by antibodies isolated from sev(epmc).pdf DeepDyve Pubget Overpricing