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10.3855/jidc.13545

http://scihub22266oqcxt.onion/10.3855/jidc.13545
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33571146!ä!33571146

suck abstract from ncbi


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pmid33571146      J+Infect+Dev+Ctries 2021 ; 15 (1): 58-68
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  • Clonal dispersion of Acinetobacter baumannii in an intensive care unit designed to patients COVID-19 #MMPMID33571146
  • Duran-Manuel EM; Cruz-Cruz C; Ibanez-Cervantes G; Bravata-Alcantara JC; Sosa-Hernandez O; Delgado-Balbuena L; Leon-Garcia G; Cortes-Ortiz IA; Cureno-Diaz MA; Castro-Escarpulli G; Velez-Resendiz JM; Bello-Lopez JM
  • J Infect Dev Ctries 2021[Jan]; 15 (1): 58-68 PMID33571146show ga
  • INTRODUCTION: SARS-CoV2 pandemic marks the need to pay attention to bacterial pathogens that can complicate the hospital stay of patients in the intensive care unit (ICU). ESKAPE bacteria which includes Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter cloacae are considered the most important, because of their close relationship with the development of ventilator-associated pneumonia (VAP). The aim of this work was to identify and characterize ESKAPE bacteria and to detect their possible clonal spread in medical devices, patients, and medical personnel of the ICU for COVID-19 patients of the Hospital Juarez de Mexico. METHODOLOGY: Genetic identification of ESKAPE bacteria was performed by analyzing the 16S rRNA gene. Resistance assays were performed according to the CLSI guidelines. Assembly of AdeABCRS operon and inhibition assays of pumps efflux in Acinetobacter baumannii isolates were performed. Associated gene involved in biofilm formation (icaA) was performed in isolates belonging to the Staphylococcus genus. Finally, typing by ERIC-PCR and characterization of mobile genetic element SCCmec were done. RESULTS: Heterogeneous distribution of ESKAPE and non-ESKAPE bacteria was detected in various medical devices, patients, and medical personnel. Acinetobacter baumannii and Staphylococcus aureus were the predominant ESKAPE members. The analysis of intergenic regions revealed an important clonal distribution of A. baumannii (AdeABCRS+). Genotyping of SCCmec mobile genetic elements and the icaA gene showed that there is no clonal distribution of S. aureus. CONCLUSIONS: Clonal spread of A. baumannii (AdeABCRS+) highlights the importance of adopting good practices for equipment disinfection, surfaces and management of COVID-19 patients.
  • |*Intensive Care Units[MESH]
  • |Acinetobacter Infections/*transmission[MESH]
  • |Acinetobacter baumannii/*isolation & purification/pathogenicity[MESH]
  • |Anti-Bacterial Agents/pharmacology[MESH]
  • |Biofilms/growth & development[MESH]
  • |COVID-19/*prevention & control[MESH]
  • |Cross Infection/microbiology/*prevention & control[MESH]
  • |Drug Resistance, Bacterial/genetics[MESH]
  • |Equipment and Supplies/microbiology[MESH]
  • |Genotype[MESH]
  • |Humans[MESH]
  • |Interspersed Repetitive Sequences[MESH]
  • |Mexico[MESH]


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