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10.7554/eLife.65365 http://scihub22266oqcxt.onion/10.7554/eLife.65365 33570490!7891930!33570490     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Elife 2021 ; 10 (ä): ä Nephropedia Template TP {{tp|p=33570490|t=2021. The Spike D614G mutation increases SARS-CoV-2 infection of multiple human cell types |pdf=|usr=}}{{33570490}} gab.com Text The Spike D614G mutation increases SARS-CoV-2 infection of multiple human cell types JO: Elife http://www.kidney.de/pget.php?&tw=1&pmid=33570490 #FOAvip A novel variant of the SARS-CoV-2 virus carrying a point mutation in the Spike protein (D614G) has recently emerged and rapidly surpassed others in prevalence. This mutation is in linkage disequilibrium with an ORF1b protein variant (P314L), making it difficult to discern the functional significance of the Spike D614G mutation from population genetics alone. Here, we perform site-directed mutagenesis on wild-type human-codon-optimized Spike to introduce the D614G variant. Using multiple human cell lines, including human lung epithelial cells, we found that the lentiviral particles pseudotyped with Spike D614G are more effective at transducing cells than ones pseudotyped with wild-type Spike. The increased transduction with Spike D614G ranged from 1.3- to 2.4-fold in Caco-2 and Calu-3 cells expressing endogenous ACE2 and from 1.5- to 7.7-fold in A549(ACE2) and Huh7.5(ACE2) overexpressing ACE2. Furthermore, trans-complementation of SARS-CoV-2 virus with Spike D614G showed an increased infectivity in human cells. Although there is minimal difference in ACE2 receptor binding between the D614 and G614 Spike variants, the G614 variant is more resistant to proteolytic cleavage, suggesting a possible mechanism for the increased transduction. Twit Text FOAVip The Spike D614G mutation increases SARS-CoV-2 infection of multiple human cell types ????Elife http://www.kidney.de/pget.php?&tw=1&pmid=33570490 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33570490 #FOAvip English Wikipedia <ref>{{Cite pmid|33570490}}</ref> The Spike D614G mutation increases SARS-CoV-2 infection of multiple human cell types #MMPMID33570490 Daniloski Z; Jordan TX; Ilmain JK; Guo X; Bhabha G; tenOever BR; Sanjana NE Elife 2021[Feb]; 10 (ä): ä PMID33570490show ga A novel variant of the SARS-CoV-2 virus carrying a point mutation in the Spike protein (D614G) has recently emerged and rapidly surpassed others in prevalence. This mutation is in linkage disequilibrium with an ORF1b protein variant (P314L), making it difficult to discern the functional significance of the Spike D614G mutation from population genetics alone. Here, we perform site-directed mutagenesis on wild-type human-codon-optimized Spike to introduce the D614G variant. Using multiple human cell lines, including human lung epithelial cells, we found that the lentiviral particles pseudotyped with Spike D614G are more effective at transducing cells than ones pseudotyped with wild-type Spike. The increased transduction with Spike D614G ranged from 1.3- to 2.4-fold in Caco-2 and Calu-3 cells expressing endogenous ACE2 and from 1.5- to 7.7-fold in A549(ACE2) and Huh7.5(ACE2) overexpressing ACE2. Furthermore, trans-complementation of SARS-CoV-2 virus with Spike D614G showed an increased infectivity in human cells. Although there is minimal difference in ACE2 receptor binding between the D614 and G614 Spike variants, the G614 variant is more resistant to proteolytic cleavage, suggesting a possible mechanism for the increased transduction. |*Mutation, Missense[MESH] |Angiotensin-Converting Enzyme 2/genetics/metabolism[MESH] |COVID-19/genetics/metabolism/*virology[MESH] |Humans[MESH] |SARS-CoV-2/genetics/*metabolism[MESH]The Spike D614G mutation increases SARS-CoV-2 infection of multiple hu(epmc).pdf DeepDyve Pubget Overpricing