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10.1021/acs.analchem.0c05059

http://scihub22266oqcxt.onion/10.1021/acs.analchem.0c05059
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33570399!ä!33570399

suck abstract from ncbi


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pmid33570399      Anal+Chem 2021 ; 93 (7): 3325-3330
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  • Rapid One-Pot Detection of SARS-CoV-2 Based on a Lateral Flow Assay in Clinical Samples #MMPMID33570399
  • Zhang C; Zheng T; Wang H; Chen W; Huang X; Liang J; Qiu L; Han D; Tan W
  • Anal Chem 2021[Feb]; 93 (7): 3325-3330 PMID33570399show ga
  • Rapid tests for pathogen identification and spread assessment are critical for infectious disease control and prevention. The control of viral outbreaks requires a nucleic acid diagnostic test that is sensitive and simple and delivers fast and reliable results. Here, we report a one-pot direct reverse transcript loop-mediated isothermal amplification (RT-LAMP) assay of SARS-CoV-2 based on a lateral flow assay in clinical samples. The entire contiguous sample-to-answer workflow takes less than 40 min from a clinical swab sample to a diagnostic result without professional instruments and technicians. The assay achieved an accuracy of 100% in 12 synthetic and 12 clinical samples compared to the data from PCR-based assays. We anticipate that our method will provide a universal platform for rapid and point-of-care detection of emerging infectious diseases.
  • |*COVID-19 Testing[MESH]
  • |COVID-19/*diagnosis[MESH]
  • |Humans[MESH]
  • |Molecular Diagnostic Techniques[MESH]
  • |Nucleic Acid Amplification Techniques[MESH]
  • |Point-of-Care Testing[MESH]
  • |SARS-CoV-2[MESH]
  • |Time Factors[MESH]


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