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10.1016/j.gendis.2021.01.008

http://scihub22266oqcxt.onion/10.1016/j.gendis.2021.01.008
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suck abstract from ncbi


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pmid33564711      Genes+Dis 2022 ; 9 (1): 275-281
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  • A peptide-based assay discriminates individual antibody response to SARS-CoV-2 #MMPMID33564711
  • Polvere I; Voccola S; Cardinale G; Fumi M; Aquila F; Parrella A; Madera JR; Stilo R; Vito P; Zotti T
  • Genes Dis 2022[Jan]; 9 (1): 275-281 PMID33564711show ga
  • SARS-CoV-2 virus is responsible for the current worldwide coronavirus disease 2019 (COVID-19) pandemic, infecting millions of people and causing hundreds of thousands of deaths. Understanding the antibody response to SARS-CoV-2 is crucial for the development of vaccines, therapeutics and public health interventions. However, lack of consistency in methods used to monitor antibody response to SARS-CoV-2 leaves some uncertainty in our fine understanding of the human antibody response mounted following SARS-CoV-2 infection. We developed a peptide-based enzyme-linked immunosorbent assay (ELISA) by selecting 7 synthetic peptides from the spike, membrane, and nucleocapsid protein sequences of SARS-CoV-2, which effectively detects the antibody response mounted by all COVID-19 convalescent tested. Strikingly, the assay shows a profound difference in antibody response among individual subjects, which may have a significant impact on disease severity. Together, our results define an efficient and specific serological assay to consistently measure the antibody response following SARS-CoV-2 infection, as well as help the design of vaccine and therapeuticals for prevention and treatment of COVID-19.
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