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Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Colloids+Surf+A+Physicochem+Eng+Asp 2021 ; 615 (ä): 126275 Nephropedia Template TP
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Nano-size dependence in the adsorption by the SARS-CoV-2 spike protein over gold colloid #MMPMID33564211
Yokoyama K; Ichiki A
Colloids Surf A Physicochem Eng Asp 2021[Apr]; 615 (ä): 126275 PMID33564211show ga
Gold nano-particles were coated with the spike protein (S protein) of SARS-CoV-2 and exposed to increasingly acidic conditions. Their responses were investigated by monitoring the surface plasmon resonance (SPR) band shift. As the external pH was gradually changed from neutral pH to pH approximately 2 the peak of the SPR band showed a significant red-shift, with a sigmoidal feature implying the formation of the gold-protein aggregates. The coating of S protein changed the surface property of the gold enough to extract the coverage fraction of protein over nano particles, Theta, which did not exhibit clear nano-size dependence. The geometrical simulation to explain Theta showed the average axial length to be a = 7. 25 nm and b =8.00 nm when the S-protein was hypothesized as a prolate shape with spiking-out orientation. As the pH value externally hopped between pH approximately 3 and pH approximately 10, a behavior of reversible protein folding was observed for particles with diameters >30 nm. It was concluded that S protein adsorption conformation was impacted by the size (diameter, d) of a core nano-gold, where head-to-head dimerized S protein was estimated for d = 80 nm and a parallel in opposite directions formation for d = 100 nm.