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10.1186/s13073-021-00839-5 http://scihub22266oqcxt.onion/10.1186/s13073-021-00839-5 33563320!7871948!33563320     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Genome+Med 2021 ; 13 (1): 21 Nephropedia Template TP {{tp|p=33563320|t=2021. CoronaHiT: high-throughput sequencing of SARS-CoV-2 genomes |pdf=|usr=}}{{33563320}} gab.com Text CoronaHiT: high-throughput sequencing of SARS-CoV-2 genomes JO: Genome Med http://www.kidney.de/pget.php?&tw=1&pmid=33563320 #FOAvip We present CoronaHiT, a platform and throughput flexible method for sequencing SARS-CoV-2 genomes (</= 96 on MinION or > 96 on Illumina NextSeq) depending on changing requirements experienced during the pandemic. CoronaHiT uses transposase-based library preparation of ARTIC PCR products. Method performance was demonstrated by sequencing 2 plates containing 95 and 59 SARS-CoV-2 genomes on nanopore and Illumina platforms and comparing to the ARTIC LoCost nanopore method. Of the 154 samples sequenced using all 3 methods, >/= 90% genome coverage was obtained for 64.3% using ARTIC LoCost, 71.4% using CoronaHiT-ONT and 76.6% using CoronaHiT-Illumina, with almost identical clustering on a maximum likelihood tree. This protocol will aid the rapid expansion of SARS-CoV-2 genome sequencing globally. Twit Text FOAVip CoronaHiT: high-throughput sequencing of SARS-CoV-2 genomes ????Genome Med http://www.kidney.de/pget.php?&tw=1&pmid=33563320 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33563320 #FOAvip English Wikipedia <ref>{{Cite pmid|33563320}}</ref> CoronaHiT: high-throughput sequencing of SARS-CoV-2 genomes #MMPMID33563320 Baker DJ; Aydin A; Le-Viet T; Kay GL; Rudder S; de Oliveira Martins L; Tedim AP; Kolyva A; Diaz M; Alikhan NF; Meadows L; Bell A; Gutierrez AV; Trotter AJ; Thomson NM; Gilroy R; Griffith L; Adriaenssens EM; Stanley R; Charles IG; Elumogo N; Wain J; Prakash R; Meader E; Mather AE; Webber MA; Dervisevic S; Page AJ; O'Grady J Genome Med 2021[Feb]; 13 (1): 21 PMID33563320show ga We present CoronaHiT, a platform and throughput flexible method for sequencing SARS-CoV-2 genomes ( 96 on Illumina NextSeq) depending on changing requirements experienced during the pandemic. CoronaHiT uses transposase-based library preparation of ARTIC PCR products. Method performance was demonstrated by sequencing 2 plates containing 95 and 59 SARS-CoV-2 genomes on nanopore and Illumina platforms and comparing to the ARTIC LoCost nanopore method. Of the 154 samples sequenced using all 3 methods, >/= 90% genome coverage was obtained for 64.3% using ARTIC LoCost, 71.4% using CoronaHiT-ONT and 76.6% using CoronaHiT-Illumina, with almost identical clustering on a maximum likelihood tree. This protocol will aid the rapid expansion of SARS-CoV-2 genome sequencing globally. |*Pandemics[MESH] |COVID-19/*genetics/virology[MESH] |Genome, Viral/*genetics[MESH] |High-Throughput Nucleotide Sequencing[MESH] |Humans[MESH] |RNA, Viral/genetics[MESH] |SARS-CoV-2/*genetics/pathogenicity[MESH]CoronaHiT: high-throughput sequencing of SARS-CoV-2 genomes (epmc).pdf DeepDyve Pubget Overpricing