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10.1016/j.xpro.2021.100357 http://scihub22266oqcxt.onion/10.1016/j.xpro.2021.100357 33558863!7859716!33558863     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       STAR+Protoc 2021 ; 2 (1): 100357 Nephropedia Template TP {{tp|p=33558863|t=2021. Expression and purification of tag-free SARS-CoV-2 RNA-dependent RNA polymerase in Escherichia coli |pdf=|usr=}}{{33558863}} gab.com Text Expression and purification of tag-free SARS-CoV-2 RNA-dependent RNA polymerase in Escherichia coli JO: STAR Protoc http://www.kidney.de/pget.php?&tw=1&pmid=33558863 #FOAvip The RNA-dependent-RNA polymerase (RdRp) from SARS-CoV-2 is an important drug target because it is responsible for viral RNA genome replication. Efficient production of recombinant RdRp is important in screening antivirals to treat COVID-19. Here, we present our protocol for expression of tag-free replication complex proteins in E. coli and subsequent purification. Despite the added complexity of multiple purification steps, our methods provide greater activity, yield at lower cost, and are faster than baculovirus expression systems. For complete details on the use and execution of this protocol, please refer to Dangerfield et al. (2020). Twit Text FOAVip Expression and purification of tag-free SARS-CoV-2 RNA-dependent RNA polymerase in Escherichia coli ????STAR Protoc http://www.kidney.de/pget.php?&tw=1&pmid=33558863 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33558863 #FOAvip English Wikipedia <ref>{{Cite pmid|33558863}}</ref> Expression and purification of tag-free SARS-CoV-2 RNA-dependent RNA polymerase in Escherichia coli #MMPMID33558863 Dangerfield TL; Huang NZ; Johnson KA STAR Protoc 2021[Mar]; 2 (1): 100357 PMID33558863show ga The RNA-dependent-RNA polymerase (RdRp) from SARS-CoV-2 is an important drug target because it is responsible for viral RNA genome replication. Efficient production of recombinant RdRp is important in screening antivirals to treat COVID-19. Here, we present our protocol for expression of tag-free replication complex proteins in E. coli and subsequent purification. Despite the added complexity of multiple purification steps, our methods provide greater activity, yield at lower cost, and are faster than baculovirus expression systems. For complete details on the use and execution of this protocol, please refer to Dangerfield et al. (2020). |*Coronavirus RNA-Dependent RNA Polymerase/chemistry/genetics/isolation & purification/metabolism[MESH] |*Recombinant Proteins/chemistry/genetics/isolation & purification/metabolism[MESH] |COVID-19/virology[MESH] |Escherichia coli/*genetics[MESH] |Humans[MESH]Expression and purification of tag-free SARS-CoV-2 RNA-dependent RNA p(epmc).pdf DeepDyve Pubget Overpricing